Summary for Simple Plex Control for Green Fluorescent Protein (GFP)
INTENDED USE AND DESCRIPTION: For use a quantitative control for the determination of Green Fluorescent Protein (GFP) concentrations in biological fluids. Controls are supplied lyophilized. Please refer to the lot specific certificate of analysis and Simple Plex Lyophilized Quality Control product insert for additional information.
The green fluorescent protein (GFP), originally discovered in the jellyfish, Aequorea victoria, is composed of 238 amino acids and has a molecular weight of 27 kDa. The molecular structure of GFP represents a cylinder, comprising 11 strands of beta-sheet outside, a single alpha helical strand containing the chromophore running through the center, and short helical segments on the ends. GFP transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Because of this property, GFP has been widely used as a tracer in various disciplines of biology research, such as fluorescence microscopy, reporter gene expression, and in vivo imaging.
Laboratory mutation of GFP generated the widely used analog GFPuv, which has brighter fluorescence and more desirable expression properties. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. This form of GFP expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395 nm and emission at 508 nm. In the presence of UV light, GFPuv emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins.
Antibodies directed against GFPuv provide additional tools for confirming GFPuv expression in cells and tissues. During immunohistochemical processing, the use of alcohols and xylene results in bleaching of GFP proteins. Biochemically, detection of GFPuv by Western blot can support the expression of the tagged protein in the target tissue of interest. GFPuv antibodies can also be employed for immuno-precipitation (IP) and ELISA experiments.
Product Specifications for Simple Plex Control for Green Fluorescent Protein (GFP)
Species
Bioprocess
Applications
Simple Plex Automated Immunoassay
Source
N/A
Preparation & Storage
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
• Store lyophilized controls at 2-8 °C. Lyophilized controls are stable for up to 12 months from date of receipt. • Reconstituted controls are stable for up to 6 months from date of preparation when stored at ≤ -70 °C. Storage at -20° C is not recommended. • Individual frozen aliquots of low and high controls are designed as single-use entities. Thaw directly before use and discard any remaining volume.
