Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.
Simple Plex Cell Activation Panel 2 Best Seller
R&D Systems, part of Bio-Techne | Catalog # ST01C-CS-007366
Simple Plex 32x4 panel containing Granzyme B, IFN-gamma 3rd Gen, Perforin, and TNF-alpha 2nd Gen with sample diluent SD13. Sample type indicated with CS (cell culture).
Key Product Details
Features
The Simple Plex Human Multianalyte Assay is for the detection of human GRANB, IFN-gamma, Perforin, and TNF-alpha in Cell Culture Supernates.
| Sample Type & Volume Required Per Well | Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL) |
| Sensitivity | GRANB 1.31 pg/mL; IFN-gamma 0.17 pg/mL; Perforin 3.14 pg/mL; TNF-alpha 0.3 pg/mL |
| Assay Range | GRANB 1.31 - 5,000 pg/mL; IFN-gamma 0.17 - 4,000 pg/mL; Perforin 3.14 - 12,000 pg/mL; TNF-alpha 0.3-1,160 pg/mL |
Species
Human
Applications
Simple Plex Automated Immunoassay
Product Summary for Simple Plex Cell Activation Panel 2
Product Specifications
| Reactivity | Human |
| Specificity | Natural and recombinant human GRANB, IFN-gamma, Perforin, and TNF-alpha. |
| Cross-reactivity | <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules. |
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent
| Intra-Assay Precision | Inter-Assay Precision | ||||
|---|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 | |
| GRANB | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 28 | 1357 | 28.7 | 1377 | |
| Standard Deviation | 1.26 | 47.1 | 2.87 | 143 | |
| CV (%) | 4.5 | 3.5 | 10 | 10.4 | |
| IFN-gamma 3rd Gen | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 21.1 | 1175 | 23.4 | 1192 | |
| Standard Deviation | 1.48 | 33.4 | 2.52 | 99.4 | |
| CV (%) | 7 | 2.8 | 10.8 | 8.3 | |
| Perforin | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 92.9 | 3901 | 90.7 | 4145 | |
| Standard Deviation | 2.07 | 74.9 | 7.41 | 371 | |
| CV (%) | 2.2 | 1.9 | 8.2 | 8.9 | |
| TNF alpha 2nd Gen | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 6.99 | 383 | 0.662 | 36.9 | |
| Standard Deviation | 0.345 | 20.6 | 7.81 | 408 | |
| CV (%) | 4.9 | 5.4 | 8.5 | 9 | |
Recovery
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % | |
|---|---|---|---|
| GRANB | EDTA Plasma (n=4) | 104 | 91-117 |
| Heparin Plasma (n=4) | 95 | 86-107 | |
| Serum (n=4) | 105 | 91-119 | |
| IFN-gamma | Cerebrospinal Fluid (n=6) | 94 | 91-98 |
| EDTA Plasma (n=4) | 89 | 88-90 | |
| Heparin Plasma (n=4) | 90 | 83-99 | |
| Serum (n=4) | 95 | 83-102 | |
Formulation, Preparation, and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Multiplex Cytokine Assay Kits
Additional Multiplex Cytokine Assay Kits Products
Product Documents for Simple Plex Cell Activation Panel 2
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Product Specific Notices for Simple Plex Cell Activation Panel 2
For research use only
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