Simple Plex Alzheimer’s Duo Panel – Ultra-Sensitive

Name: Simple Plex Alzheimer’s Duo Panel – Ultra-Sensitive
Brand: ProteinSimple
SKU: SPCKC-PS-015464

ProteinSimple, part of Bio-Techne | Catalog # SPCKC-PS-015464

COA

Simple Plex 32x2 panel containing Amyloid Beta (aa1-42) and pTau (217) ALZpath with sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 3-5 days. Sample type indicated with PS (Plasma/Serum).

Key Product Details

Features:

The Simple Plex Human Multianalyte Panel, Alzheimer’s Duo Panel – Ultra Sensitive, is for the detection of Ultra-Sensitive human Amyloid Beta (aa1-42) and pTau (217) ALZpath in serum and plasma (EDTA/Heparin).

Sample Type & Volume Required Per Well	Cell Culture Supernates (40 uL), Serum (40 uL), EDTA Plasma (40 uL), Heparin Plasma (40 uL), Cerebrospinal Fluid (40 uL)
Sensitivity	Amyloid Beta (aa1-42) 0.22 pg/mL; pTau (217) 0.040 pg/mL
Assay Range	Amyloid Beta (aa1-42) 0.92 – 1,409 pg/mL; pTau (217) 0.10 – 900 pg/mL

Technical Data
Citations

Summary for Simple Plex Alzheimer’s Duo Panel – Ultra-Sensitive

Product Specifications

Reactivity	Human
Specificity	Natural and recombinant human Amyloid Beta (aa1-42) and pTau (217) ALZpath.
Cross-reactivity	12.5 ng/mL. Human Amyloid β (aa1-42) interferes at concentrations > 6.25 ng/mL.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

		Intra-Assay Precision		Inter-Assay Precision
	Sample	1	2	1	2
Amyloid Beta (aa1-42)	n	16	16	95	93
	Mean (pg/mL)	12.1	534	11.5	557
	Standard Deviation	0.696	23.9	1.26	59.9
	CV (%)	5.8	4.5	10.9	10.8
pTau (217) ALZpath	n	16	16	101	99
	Mean (pg/mL)	7.92	363	6.27	338
	Standard Deviation	0.324	11.5	0.648	32.9
	CV (%)	4.1	3.2	10.3	9.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

	Sample Type	Average % Recovery	Range %
pTau (217) ALZpath	EDTA Plasma (n=4)	73	66-80
	Heparin Plasma (n=5)	85	76-98
	Serum (n=4)	90	81-101
Amyloid Beta (aa1-42)	Recovery was not necessary as Amyloid β (aa1-42) shows 5 points of natural linearity.

Product Specifications for Simple Plex Alzheimer’s Duo Panel – Ultra-Sensitive

Species

Human

Preparation & Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Simple Plex Alzheimer’s Duo Panel – Ultra-Sensitive

ProteinSimple, part of Bio-Techne | Catalog # SPCKC-PS-015464

Key Product Details

Summary for Simple Plex Alzheimer’s Duo Panel – Ultra-Sensitive

Product Specifications

Precision

Recovery

Product Specifications for Simple Plex Alzheimer’s Duo Panel – Ultra-Sensitive

Preparation & Storage

Product Documents for Simple Plex Alzheimer’s Duo Panel – Ultra-Sensitive

COA

Certificate of Analysis

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