Non-Human Primate VEGF-A Cartridge

Name: Non-Human Primate VEGF-A Cartridge
Brand: ProteinSimple
SKU: SPCKB-NP-013073

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-013073

Product Datasheet / COA

Simple Plex NHP VEGF-A assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.54 pg/mL
Assay Range:	3.84 - 5,867 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Non-Human Primate VEGF-A Cartridge Summary:	Simple Plex assay is for the detection of non-human primate Vascular Endothelial Growth Factor A (VEGF-A or VEGF) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Non-Human Primate VEGF-A Cartridge

Technical Data
Citations

Product Specifications for Non-Human Primate VEGF-A Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Non-human Primate
Specificity	Natural and recombinant VEGF-A.
Cross-reactivity	Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	38	34
Mean (pg/mL)	44.8	2008	44.1	2096
Standard Deviation	1.6	45.3	4.13	159
CV%	3.6	2.3	9.4	7.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	106	101-110

Linearity

Samples containing and/or spiked with high concentrations of non-human primate VEGF-A were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Non-Human Primate VEGF-A Cartridge

	Simple Plex Non-Human Primate VEGF-A Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Non-Human Primate VEGF-A Assay. Assay range is 3.84-5867 pg/mL.

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF

VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.

Item	Value
Long Name	Vascular Endothelial Growth Factor
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names	MVCD1, VAS, VEGF, VEGF-A, VEGFA, VEGFMGC70609, VPF, VPFvascular endothelial growth factor, Vascular permeability factor, Vasculotropin, vascular endothelial growth factor A

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Non-Human Primate VEGF-A Cartridge

Product Datasheets

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COA

Protocols & Manuals

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