Troponin I Type 3 (cardiac) Antibody - Azide Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20734
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (92%), Canine (95%), Feline (94%), Porcine (93%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Full length human Troponin I Type 3 (cardiac) Recombinant protein.
Reactivity Notes
Cat (94%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Troponin I Type 3 (cardiac) Antibody - Azide Free
![Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-20734]](https://resources.bio-techne.com/images/products/Troponin-I-Type-3-cardiac-Antibody-Immunohistochemistry-Paraffin-NBP2-20734-img0012.jpg "Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-20734]")
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-20734]
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-20734] - Troponin I Type 3 (cardiac) antibody detects Troponin I Type 3(cardiac) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Green: Troponin I Type 3 (cardiac) stained by Troponin I Type 3 (cardiac) antibody diluted at 1:250. Red: beta Actin, a cytoskeleton marker, stained by beta Actin antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Troponin I Type 3 (cardiac) Antibody [NBP2-20734] -
Western Blot: Troponin I Type 3 (cardiac) Antibody [NBP2-20734] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Troponin I Type 3 (cardiac) antibody [N1C3] (NBP2-20734) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry: Troponin I Type 3 (cardiac) Antibody [NBP2-20734] -
Troponin I (cardiac) antibody [N1C3] detects Troponin I (cardiac) protein at cytosol on rat heart by immunohistochemical analysis.Sample: Paraffin-embedded rat heart.
Troponin I (cardiac) antibody [N1C3] (NBP2-20734) dilution: 1:500.
br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Applications for Troponin I Type 3 (cardiac) Antibody - Azide Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Format
Azide Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Troponin I/TNNI3
Long Name
Troponin I3, Cardiac Type
Alternate Names
CMD2A, CTnI, RCM1, TNNC1, TNNI3,
Gene Symbol
TNNI3
UniProt
Additional Troponin I/TNNI3 Products
Product Documents for Troponin I Type 3 (cardiac) Antibody - Azide Free
Product Specific Notices for Troponin I Type 3 (cardiac) Antibody - Azide Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.