Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Recombinant Viral wnvNS1 His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10945-SE

Catalog #
Availability
Size / Price
Qty
Loading...
10945-SE-100

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived viral wnvNS1 Protein protein
Asp1-Ala352, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp1

Predicted Molecular Mass

40 kDa

SDS-PAGE

52-62 kDa, under reducing conditions.

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Viral wnvNS1 His-tag Protein, CF

Recombinant Viral wnvNS1 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Viral wnvNS1 His-tag Protein (Catalog # 10945-SE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 52-62 kDa.

Formulation, Preparation and Storage

10945-SE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: wnvNS1 Protein

The West Nile Virus [WNV] is a neurotropic human pathogen. This virus belongs to the flavivirus genus which is in the Flaviviridae family along with the Dengue virus (DENV), Yellow fever virus (YFV), Japanese encephalitis virus (JEV), and Zika virus. The West Nile Virus is a mosquito-borne virus and affects many other animals besides humans, such as birds, reptiles and other mammals (1). The West Nile Virus is an enveloped virion that contains a positive single stranded RNA. The viral RNA is translated to produce 3 structural and 7 nonstructural proteins. The structural proteins are capsid (C), envelope (E), and precursor membrane proteins (prM) and the nonstructural proteins include NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5. The structural proteins are encoded at the 5' end of the RNA strand and serve the function of virus entry and encapsulation of the genome (2). WNV NS1 protein is a glycoprotein ~50 kDa. It shares 55.7% and 54.7% homology with NS1 from DENV2 and Zika virus. NS1 exists as a monomer, dimer, and hexamer, depending on its localization (3-5). Glycosylation is critical for its functions (6). Although no known role in viron assembly, NS1 is important for virulence and viral replication (7). Intracellular NS1 is reported to interact with various host proteins, such as 60S ribosome subunit, hnRNP C1/C2 and hnRNP K to assist virus replication (8-10). TLR3 receptor is reported to mediate WNV entry into the brain (11). Secreted and cell surface associated NS1 is highly immunogenic, making it an important biomarker and vaccine target (12-14).

References

  1. Lindenbach, B.D. et al. (2003) Adv. Virus Res. 59:23
  2. Rastogi, M. et al. (2016) Virol. J. 13:131.
  3. Winkler, G. et al. (1988) Virology. 162:187.
  4. Macdonald, J. et al. (2005) J. Virol. 79:13924.
  5. Akey, D.L. et al. (2014) Science 343:881.
  6. Somnuke, P. et al. (2011) Virology 413:253.
  7. Akey, D.L. et al. (2015) Bioessays 37:489.
  8. Cervantes-Salazar, M. et al. (2015) Virology 484:113.
  9. Noisakran, S. et al. (2008) Biochem Biophys Res Commun. 372:67.
  10. Kanlaya, R. et al. (2010) Mol Biosyst. 6:795.
  11. Wang, T. et al. (2004) Nat Med. 10:1366.
  12. Watterson, D. et al. (2016) Antiviral Res. 130:7.
  13. Sun, E. et al. (2013) Vet Microbiol. 166:145.
  14. Wu, S.F. et al. (2003) Vaccine. 21:3919.

Long Name

West Nile Virus Non-structural Protein NS1

Entrez Gene IDs

912267 (Viral)

Gene Symbol

POLY

UniProt

Additional wnvNS1 Protein Products

Product Documents for Recombinant Viral wnvNS1 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Viral wnvNS1 His-tag Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=