Recombinant Rat Neurofascin Protein, CF

Name: Recombinant Rat Neurofascin Protein, CF
Brand: R&D Systems
SKU: 3235-NF

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3235-NF

Citations (3)

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Source:	NS0
Accession #:	NP_446361
Applications:	Bioactivity

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Product Specifications for Recombinant Rat Neurofascin Protein, CF

Source	Mouse myeloma cell line, NS0-derived rat Neurofascin protein Ile25-Ala1031, with a C-terminal 6-His tag
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ile25
Predicted Molecular Mass	114.7 kDa
SDS-PAGE	135-140 kDa, reducing conditions
Activity	Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that had been pre-coated with 50 µL/well of the rrNeurofascin solution at 10-15 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3235-NF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Neurofascin

Neurofascin, a type I transmembrane glycoprotein, is a member of the L1 family of cell adhesion molecules (CAMs) (1-4). L1CAM family members are composed of an extracellular domain (ECD) that contains six immunoglobulin (Ig)-like domains and multiple fibronectin type III repeats, followed by transmembrane and cytoplasmic domains (2, 4, 5). Multiple isoforms of Neurofascin, including NF155, NF166, NF180, and NF186, can be generated by alternative splicing that differ in the combination of fibronectin type III repeats, as well as in the presence of a proline-, alanine-, and threonine-rich segment (PAT domain) located just after the fourth fibronectin type III repeat (4). This recombinant rat Neurofascin protein corresponds to isoform NF155 and shares 98% amino acid sequence identity with comparable regions of human and mouse Neurofascin. In rats, NF155 is transiently expressed by oligodendrocytes and Schwann cells during axon myelination (6, 7). NF155 clusters in paranodal regions of oligodendroglia and binds to the Caspr-Contactin complex located on the adjacent axon to form and stabilize paranodal axoglial junctions (8-11). It has been suggested that the ECD of NF155 must be cleaved from oligodendroglia membranes to form and/or stabilize the paranodal structure (12). NF155 has also been shown to promote neuronal adhesion and neurite outgrowth in rats and chickens (12-14). Alterations in NF155 expression have been associated with multiple sclerosis (15).

Item	Value
References	Rathjen, F.G. et al. (1987) Cell 51:841. Volkmer, H. et al. (1992) J. Cell Biol. 118:149. Sherman, D.L. and P.J. Brophy (2005) Nat. Rev. Neurosci. 6:683. Kriebel, M. et al. (2012) Int. J. Biochem. Cell Biol. 44:694. Liu, H. et al. (2011) J. Biol. Chem. 286:797. Collinson, J.M. et al. (1998) Glia 23:11. Basak, S. et al. (2007) Dev. Biol. 311:408. Charles, P. et al. (2002) Curr. Biol. 12:217. Schafer, D.P. et al. (2004) J. Neurosci. 24:3176. Maier, O. et al. (2005) Mol. Cell. Neurosci. 28:390. Sherman, D.L. et al. (2005) Neuron 48:737. Maier, O. et al. (2006) Exp. Cell Res. 312:500. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059. Koticha, D. et al. (2005) Mol. Cell. Neurosci. 30:137. Howell, O.W. et al. (2006) Brain 129:3173.
Entrez Gene IDs	23114 (Human); 269116 (Mouse); 116690 (Rat)
Alternate Names	DKFZp686P2250, KIAA0756FLJ46866, NF, NFASC, NRCAML, Neurofascin, neurofascin homolog, neurofascin homolog (chicken)

Citations for Recombinant Rat Neurofascin Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat Neurofascin Protein, CF include:

Applications: Bioassay

Katsunori Ikewaki (2016), IgG4 anti-neurofascin155 antibodies in chronic inflammatory demyelinating polyradiculoneuropathy: Clinical significance and diagnostic utility of a conventional assay J. Neuroimmunol., 0(0).
PMID: 27852440
Species: Human
Sample Types: Serum
Applications: ELISA (Capture)

Pruss H et al. (2011), Neurofascin as target of autoantibodies in Guillain-Barre syndrome. Brain, 134(0):e173; author.
PMID: 21278083
Species: Human
Sample Types: Serum
Applications: ELISA (Capture)

Mathey EK et al. (2007), Neurofascin as a novel target for autoantibody-mediated axonal injury. J. Exp. Med., 204(10):2363-72.
PMID: 17846150

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Recombinant Rat Neurofascin Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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