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Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF

Catalog # 11261-GT | R&D Systems, Inc. a Bio-Techne Brand
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11261-GT-020

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived porcine alpha3GalT/GGTA1 protein
Glu23-Ile371, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu23

Predicted Molecular Mass

42 kDa

SDS-PAGE

39-48 kDa, under reducing conditions.

Activity

Measured by its ability to transfer galactose from UDP-galactose to alpha-Lactose.
The specific activity is >400 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF

Recombinant Porcine alpha3GalT/GGTA1 His-tag Enzyme Activity Diagram.

Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein (Catalog # 11261-GT) introduces an alpha1-3 linked galactose residue to terminal lactosamine (LN) structure and generates alpha-Gal epitope (boxed). alpha-Gal is ubiquitously presented in non-primate mammals but absent in humans and is responsible for hyperacute rejection of organs transplanted from alpha-gal donors. The enzyme shows reduced activity on lactose, which is the basis for the current enzyme activity assay.

Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein (Catalog # 11261-GT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 39‑48 kDa.

Formulation, Preparation and Storage

11261-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: alpha3GalT/GGTA1

GGTA1, also designated as alpha3GalT, is an alpha­1,3 galactosyltransferase that catalyzes the transfer of galactose from UDP­ alpha­D­galactose to various glycoconjugates to form non­reducing terminal alpha­1,3­linked galactosyl moieties, or alpha-Gal epitope (1). This enzyme is expressed in many mammalian species but is absent from humans, apes, and Old World monkeys due to the mutational inactivation of the gene (2). Therefore, humans do not have alpha-Gal on glycoconjugates, but produce large amounts of antibody to this epitope, resulting from exposure to alpha-Gal containing antigens (3). GGTA1 is of great medical interest because the immune responses may be enhanced when selected targets are decorated with alpha-Gal structures (4). Additionally, the presence of anti-­ alpha-­Gal antibodies is a barrier to xenotransplantation of organs. Recombinant pig GGTA1 may provide easy access to a wide spectrum of alpha-Gal epitopes and their derivatives to support studies on xenotransplantation and other pharmaceutical research (5, 6). Structurally and mechanistically GGTA1 is a model for several homologous glycosyltransferases that differ in donor and acceptor substrate specificity, including the histo blood group A and B glycosyltransferases, Forssman glycolipid synthase, and isogloboside 3 synthase (7).

References

  1. Larsen R.D. et al. (1989) Proc. Natl. Acad. Sci. U.S.A. 86:8227. 
  2. Joziasse, D.H. et al. (1992) J. Biol. Chem. 267:5534. 
  3. Wigglesworth K.M. et al. (2011) J. Immunol. 186:4422. 
  4. Dequchi, T. et al. (2010) Cancer Res. 70:5259. 
  5. Anraku, K. et al. (2017) Org. Biomol. Chem. 15:2979. 
  6. Galili, U. (2021) Front. Mol. Biosci. 8:746883. 
  7. Breton, C. et al. (2005) Glycobiology 16:29R.  

Long Name

alpha 1,3-Galactosyltransferase

Alternate Names

alpha Gal, GALT, GGTA1, Glt6d1

Entrez Gene IDs

14594 (Mouse); 246766 (Rat); 396733 (Porcine)

Gene Symbol

GGTA1

UniProt

Additional alpha3GalT/GGTA1 Products

Product Documents for Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF

For research use only

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