Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF New

Name: Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF
Brand: R&D Systems
SKU: 11261-GT

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 11261-GT

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Key Product Details

Source:	CHO
Accession #:	P50127-1
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF

Product Specifications for Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived porcine alpha3GalT/GGTA1 protein Glu23-Ile371, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Glu23
Predicted Molecular Mass	42 kDa
SDS-PAGE	39-48 kDa, under reducing conditions.
Activity	Measured by its ability to transfer galactose from UDP-galactose to alpha -Lactose. The specific activity is >400 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF

	Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein (Catalog # 11261-GT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 39‑48 kDa.
	Recombinant Porcine alpha3GalT/GGTA1 His-tag Enzyme Activity Diagram. Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein (Catalog # 11261-GT) introduces an alpha 1-3 linked galactose residue to terminal lactosamine (LN) structure and generates alpha -Gal epitope (boxed). alpha -Gal is ubiquitously presented in non-primate mammals but absent in humans and is responsible for hyperacute rejection of organs transplanted from alpha -gal donors. The enzyme shows reduced activity on lactose, which is the basis for the current enzyme activity assay.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11261-GT

Formulation	Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background:

GGTA1, also designated as alpha 3GalT, is an alpha 1,3 galactosyltransferase that catalyzes the transfer of galactose from UDP alpha Dgalactose to various glycoconjugates to form nonreducing terminal alpha 1,3linked galactosyl moieties, or alpha -Gal epitope (1). This enzyme is expressed in many mammalian species but is absent from humans, apes, and Old World monkeys due to the mutational inactivation of the gene (2). Therefore, humans do not have alpha -Gal on glycoconjugates, but produce large amounts of antibody to this epitope, resulting from exposure to alpha -Gal containing antigens (3). GGTA1 is of great medical interest because the immune responses may be enhanced when selected targets are decorated with alpha -Gal structures (4). Additionally, the presence of anti- alpha -Gal antibodies is a barrier to xenotransplantation of organs. Recombinant pig GGTA1 may provide easy access to a wide spectrum of alpha -Gal epitopes and their derivatives to support studies on xenotransplantation and other pharmaceutical research (5, 6). Structurally and mechanistically GGTA1 is a model for several homologous glycosyltransferases that differ in donor and acceptor substrate specificity, including the histo blood group A and B glycosyltransferases, Forssman glycolipid synthase, and isogloboside 3 synthase (7).

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References	Larsen R.D. et al. (1989) Proc. Natl. Acad. Sci. U.S.A. 86:8227. Joziasse, D.H. et al. (1992) J. Biol. Chem. 267:5534. Wigglesworth K.M. et al. (2011) J. Immunol. 186:4422. Dequchi, T. et al. (2010) Cancer Res. 70:5259. Anraku, K. et al. (2017) Org. Biomol. Chem. 15:2979. Galili, U. (2021) Front. Mol. Biosci. 8:746883. Breton, C. et al. (2005) Glycobiology 16:29R.

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Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF New

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF

Scientific Data Examples for Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

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Background:

Citations for Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF

Product Documents for Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF

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