Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 11261-GT
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived porcine alpha3GalT/GGTA1 protein
Glu23-Ile371, with a C-terminal 6-His tag
Glu23-Ile371, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu23
Predicted Molecular Mass
42 kDa
SDS-PAGE
39-48 kDa, under reducing conditions.
Activity
Measured by its ability to transfer galactose from UDP-galactose to alpha-Lactose.
The specific activity is >400 pmol/min/μg, as measured under the described conditions.
The specific activity is >400 pmol/min/μg, as measured under the described conditions.
Scientific Data Images for Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF
Recombinant Porcine alpha3GalT/GGTA1 His-tag Enzyme Activity Diagram.
Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein (Catalog # 11261-GT) introduces an alpha1-3 linked galactose residue to terminal lactosamine (LN) structure and generates alpha-Gal epitope (boxed). alpha-Gal is ubiquitously presented in non-primate mammals but absent in humans and is responsible for hyperacute rejection of organs transplanted from alpha-gal donors. The enzyme shows reduced activity on lactose, which is the basis for the current enzyme activity assay.Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein (Catalog # 11261-GT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 39-48 kDa.Formulation, Preparation and Storage
11261-GT
Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: alpha3GalT/GGTA1
References
- Larsen R.D. et al. (1989) Proc. Natl. Acad. Sci. U.S.A. 86:8227.
- Joziasse, D.H. et al. (1992) J. Biol. Chem. 267:5534.
- Wigglesworth K.M. et al. (2011) J. Immunol. 186:4422.
- Dequchi, T. et al. (2010) Cancer Res. 70:5259.
- Anraku, K. et al. (2017) Org. Biomol. Chem. 15:2979.
- Galili, U. (2021) Front. Mol. Biosci. 8:746883.
- Breton, C. et al. (2005) Glycobiology 16:29R.
Long Name
alpha 1,3-Galactosyltransferase
Alternate Names
alpha Gal, GALT, GGTA1, Glt6d1
Gene Symbol
GGTA1
UniProt
Additional alpha3GalT/GGTA1 Products
Product Documents for Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF
Product Specific Notices for Recombinant Porcine alpha3GalT/GGTA1 His-tag Protein, CF
For research use only
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