Recombinant P. multocida Hyaluronan Synthase/HAS Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9585-GT

R&D Systems, part of Bio-Techne
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9585-GT-050
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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

E. coli

Accession #

Q7BLV3

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Hyaluronan Synthase Proteins and Enzymes »

Product Specifications

Source

E. coli-derived p. multocida Hyaluronan Synthase protein
Met1-Ile703, with C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

81 kDa

SDS-PAGE

74 kDa, reducing conditions

Activity

Measured by its ability to transfer GlcNAc and GlcA from donor substrates to hyaluronan.
The specific activity is >80 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

9585-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Hyaluronan Synthase

Hyaluronan (HA) is a polysaccharide chain composed of repeating beta4GlcUA-beta 3GlcNAc disaccharide units with molecular weights generally ranging from ~104 to 107 Da in vertebrates and bacteria (1, 2). In animals, HA plays structural, recognition and signaling roles. Certain pathogenic bacteria, namely Streptococcus Group A and C and Pasteurella multocida Type A, utilize extracellular HA polysaccharide capsules to avoid host defenses and to increase virulence. It is now recognized that HA of different sizes can have dramatically different effects on cellular behavior and growth (3, 4), and vertebrates may be able to control HA size in vivo by differential expression of biosynthetic enzymes (5). The Pasteurella multocida HA synthase enzyme, pmHAS, catalyzes the synthesis of HA polymer by alternative addition of GlcNAc and GlcA residues to the nonreducing terminus of HA using the donor substrates UDP-GlcNAc and UDP-GlcA (6). The enzyme can also elongate exogenous HA oligosaccharide acceptors in vitro (7, 8), therefore can be used for non-reducing end labeling of HA. The enzymatic activity of pmHAS was determined using a phosphatase-coupled assay (9).

References

  1. DeAngelis, P. L. (2002) Glycobiology 12:9R.
  2. Toole, B. P. (2001) Semin Cell. Dev. Biol. 12:79.
  3. McKee, C. M. et al. (1996) J. Clin. Invest. 98:2403.
  4. Tian, X. et al. (2013) Nature  499:346.
  5. Spicer, A. P. et al. (1998) J. Biol. Chem. 273:25117.
  6. Jing, W. and DeAngelis, P. L. (2004) J. Biol. Chem. 279:42345
  7. Weigel, P.H. and DeAngelis, P. L. (2007) J. Biol. Chem. 282:36777
  8. DeAngelis, P. L. (1999) J. Biol. Chem. 274:26557.
  9. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Alternate Names

HA Synthetase, HAS, Hyaluronic Acid Synthase, Hyaluronic Acid Synthetase

UniProt

Q7BLV3

Additional Hyaluronan Synthase Products

Product Documents for Recombinant P. multocida Hyaluronan Synthase/HAS Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant P. multocida Hyaluronan Synthase/HAS Protein, CF

For research use only

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