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Recombinant Mouse VSTM4 His-tag Protein, CF

Catalog # 10210-VT | R&D Systems, Inc. a Bio-Techne Brand
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10210-VT-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse VSTM4 protein
Leu24-Tyr179, with C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu24

Predicted Molecular Mass

20 kDa

SDS-PAGE

30-44 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect is 0.5-5 μg/mL.

Scientific Data Images for Recombinant Mouse VSTM4 His-tag Protein, CF

Recombinant Mouse VSTM4 His-tag Protein Bioactivity

Recombinant Mouse VSTM4 His-tag Protein Bioactivity

Recombinant Mouse VSTM4 (Catalog # 10210-VT) inhibits anti-CD3 antibody-induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-5 μg/mL.
Recombinant Mouse VSTM4 His-tag Protein SDS-PAGE

Recombinant Mouse VSTM4 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse VSTM4 His-tag (Catalog # 10210-VT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-44 kDa.

Formulation, Preparation and Storage

10210-VT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSTM4

V-set and transmembrane domain-containing protein 4 (VSTM4) is a single-pass type I membrane protein in the immunoglobulin superfamily. Mouse VSTM4 is synthesized as a 319 amino acid (aa) precursor that contains a 23 aa signal sequence, a 156 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 119 aa cytoplasmic tail. In humans, part of the extracellular region is cleaved into a 50 aa secreted peptide (aa 55-104) compared to mouse, which is cleaved into a 49 aa peptide (aa 55-103) (1). Because of its role in enhancing L-type voltage-gated calcium channel (L-VGCC) currents in photoreceptors, this peptide was named peptide Lv (1). Peptide Lv is expressed in the central nervous system and a variety of organs including spleen, intestine, retina, and lung (1, 2). The peptide may have possible roles in regulating the cardiovascular system and L-VGCC dependent neural plasticity (1, 2). Human VSTM4 gene is located on chromosome 10, which may be linked to late-onset Alzheimer's disease (3). Down-regulation of VSTM4 increased tamoxifen sensitivity and suppressed growth in cultured breast cancer cells (4). Within the ECD, mouse VSTM4 shares 87% and 96% aa sequence identity with human and rat VSTM4, respectively. The biological functions of VSTM4 remain unknown. Our in-house data show that mouse VSTM4 inhibits anti-CD3 induced IL-2 secretion by Human T Cells.

References

  1. Shi, L. et al. (2012) PLoS. 7:e43091.
  2. Shi, L. et al. (2015) Biochim. Biophys. Acta. 1853:1154.
  3. Grupe, A. et al. (2006) Am. J. Hum. Genet. 78:78.
  4. Mendes-Pereira, A. et al. (2012) Proc. Natl. Acad. Sci. 109:2730.

Long Name

V-Set And Transmembrane Domain Containing 4

Alternate Names

C10orf72

Entrez Gene IDs

196740 (Human); 320736 (Mouse); 361112 (Rat)

Gene Symbol

VSTM4

UniProt

Product Documents for Recombinant Mouse VSTM4 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse VSTM4 His-tag Protein, CF

For research use only

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