Recombinant Mouse TSG-6 Protein, CF

Catalog # 2326-TS | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
2326-TS-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Accession #

O08859

Source

NS0

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TSG-6 protein
Trp18-Leu275, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp18

Predicted Molecular Mass

29.8 kDa

SDS-PAGE

39 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized rmTSG-6 can bind biotinylated Hyaluronan with an estimated KD

Formulation, Preparation and Storage

2326-TS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSG-6

TSG-6 (TNF-stimulated gene 6; also named TNFIP6) is a secreted, 35 - 39 kDa group A member of the LINK-Module superfamily of proteins (1 - 4). Mouse TSG-6 is synthesized as a 275 amino acid (aa) precursor. It contains a 17 aa signal sequence and a 258 aa mature region (5, 6). The mature region has an N-terminal link module (aa 36 - 129) and a C-terminal CUB (C1s/C1r; urchin embryonic growth factor; BMP1) domain (aa 135 - 246). Link modules bind hyaluronan (HA) and participate in extracellular matrix (ECM) assembly (7). Mature mouse TSG-6 shares 97%, 94% and 94% aa identity with rat, human and canine TSG-6, respectively. Cells reported to express TGF-6 include activated fibroblasts, synoviocytes, chondrocytes, neutrophils, proximal tubular epithelium, bronchial epithelium, endothelium, and visceral plus vascular smooth muscle (2, 8). TSG-6 has multiple functions, many of which involve the ECM. It is suggested to stabilize HA-rich ECM. It does so by serving as an intermediary, or as a link between the individual subunits of the extracellular decameric pentraxin 3 and the surrounding hyaluronan matrix (9). It also provides structure and organization to hyaluronan. This is accomplished by a TSG-6 mediated transfer of an 80 - 85 kDa protein subunit from I alphaI (inter-alpha -inhibitor) to HA. I alphaI is a four-component, 225 kDa serine protease inhibitor. It contains a protease inhibitor subunit (bikunin), two independent, accompaning protein chains (HC1 and HC2), and a short chondroitin sulfate linking moiety. TSG-6 is a catalyst for the removal and transient binding of either HC chain. Each chain is subsequently transferred and covalently-linked to the surrounding HA. This provides substance and reinforcement to the ECM (1, 2, 10, 11, 12). This disassembly of I alphaI also leads to free bikunin, which in the “free” state becomes a potent inhibitor of serine proteases (8).

References

  1. Milner, C.M. et al. (2006) Biochem. Soc. Trans. 34:446.
  2. Milner, C.M. and A.J. Day (2003) J. Cell Sci. 116:1863.
  3. Wisnieewski, H-G. and J. Vilcek (2004) Cytokine Growth Factor Rev. 15:129.
  4. Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
  5. Fulop, C. et al. (1997) Gene 202:95.
  6. Fulop, C. et al. (2003) Development 130:2253.
  7. Kohda, D. et al. (1996) Cell 86:767.
  8. Forteza, R. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:20.
  9. Salustri, A. et al. (2003) Development 131:1577.
  10. Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
  11. Sanggaard, K.W. et al. (2006) Biochemistry 45:7661.
  12. Sanggaard, K.W. et al. (2005) J. Biol. Chem. 280:11936.

Long Name

Tumor Necrosis Factor-stimulated Gene Sequence 6

Alternate Names

TNFAIP6, TSG6

Entrez Gene IDs

7130 (Human); 21930 (Mouse)

Gene Symbol

TNFAIP6

UniProt

O08859

Product Documents for Recombinant Mouse TSG-6 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse TSG-6 Protein, CF

For research use only

Citations for Recombinant Mouse TSG-6 Protein, CF (9)

Showing  1 - 9 of 9 publications Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse TSG-6 Protein, CF include:

There are no citations that match your criteria.

Showing  1 - 9 of 9 publications Showing All

Customer Reviews for Recombinant Mouse TSG-6 Protein, CF (1)

5 out of 5
1 customer rating
5 stars
100%
4 stars
0%
3 stars
0%
2 stars
0%
1 star
0%
Showing  1 - 1 of 1 review Showing All
Filter By:
  • Recombinant Mouse TSG-6 Protein, CF
    Name: jayeeta giri
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 08/02/2019

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Setting the standard in quality research reagents