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Recombinant Mouse TrkA Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10235-TK

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10235-TK-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TrkA protein
Mouse TrkA
(Ala34 - Pro418)
Accession # Q3UFB7
IEGRMDP Mouse IgG2a
(Glu98 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala34

Predicted Molecular Mass

69 kDa

SDS-PAGE

100 - 125 kDa

Activity

Measured by its ability to inhibit NGF-induced proliferation of TF‑1 human erythroleukemic cells.
The ED50 for this effect is 4-24 ng/mL in the presence of 2 ng/mL of Recombinant Mouse beta‑NGF (Catalog # 1156-NG).

Scientific Data Images for Recombinant Mouse TrkA Fc Chimera Protein, CF

Recombinant Mouse TrkA Fc Chimera Protein Bioactivity

Recombinant Mouse TrkA Fc Chimera Protein Bioactivity

Recombinant Mouse TrkA Fc Chimera (Catalog # 10235-TK) inhibits NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 4-24 ng/mL in the presence of 2 ng/mL of Recombinant Mouse beta-NGF (Catalog # 1156-NG).
Recombinant Mouse TrkA Fc Chimera Protein SDS-PAGE

Recombinant Mouse TrkA Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse TrkA Fc Chimera (Catalog # 10235-TK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 100-125 kDa and 200-250 kDa, respectively.

Formulation, Preparation and Storage

10235-TK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkA

Tropomyocin receptor kinase A (TrkA), also named Neurotrophic tyrosine kinase receptor type 1 (NTRK3) is a member of a nerve growth factor tyrosine kinase receptor family. There are three members of the Trk family, TrkA, TrkB and TrkC, and they bind a group of structurally related, secreted proteins termed neurotrophins, which play an important role in the development and function of the nervous system. The Trk family shares a conserved structural motif consisting of two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, a single transmembrane domain and an intracellular tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). Mature mouse TrkA consists of a 383 amino acid (aa) extracellular domain (ECD) which shares 79% and 93% aa identity with human and rat TrkA, respectively. Each Trk family member exhibits different ligand specificities: TrkA binds NGF and NT-3, TrkB binds BDNF, NT-3 and NT-4/5, and TrkC only binds NT-3 (1, 2). The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family receptors (1, 2). P75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low-affinity to transduce cellular signaling pathways that synergize or antagonize those activated by the Trk receptors. Several TrkA isoforms exist, two of which differ only by a 6-amino acid insertion in their extracellular domain (3). The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA is expressed mainly in non-neuronal tissues (3). Trk receptor interactions with NGF play major roles in the development of the sympathetic nervous system, and TrkA, specifically, is essential to the survival of sympathetic neurons in vivo (4). NGF activates retrograde transport of TrkA endosomes for association with actin-modulatory proteins to promote F-actin disassembly, enabling their maturation into transport-competent signaling endosomes (1). Inhibition of the Trk receptors may have several therapeutic implications (5). Injection of a TrkA inhibitor in patients with knee osteoarthritis resulted in sustained pain improvement in a single center trial (6). Another inhibitor showed a significant reduction in psoriatic pruritus, which occurs via a Trka-dependent mechanism (7).

References

  1. Harrington, A. et al. (2011) Cell. 146(3):421.
  2. Smeyne, R. et al. (1994) 368(6468):246.
  3. Barker, P. et al. (1992) 268(20):15150.
  4. Fagan, A. et al. (1996) J Neurosci. 16(19):6208.
  5. Yan, W. et al. (2019) J Med. Chem. 62(4):1731.
  6. Krupka, E. et al. (2019) Osteoarthr Cartil. DOI: 10:1016.
  7. Yosipovitch, R. et al. (2015) Acta Derm Venereol. 95.

Long Name

Neurotrophic Tyrosine Kinase Receptor A

Alternate Names

NTRK-1, NTRK1

Entrez Gene IDs

4914 (Human); 18211 (Mouse); 59109 (Rat); 102129364 (Cynomolgus Monkey)

Gene Symbol

NTRK1

UniProt

Additional TrkA Products

Product Documents for Recombinant Mouse TrkA Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse TrkA Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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