Recombinant Mouse TLR6 Fc Chimera Protein, CF

Catalog # 1533-TR | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

BAA78632

Source

Sf 21 (stably transfected)

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse TLR6 protein
Mouse TLR6
(Leu43-Asp594)
Accession # BAA78632		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu43

Predicted Molecular Mass

89.3 kDa (monomer)

SDS-PAGE

100 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized peptidoglycan at 10 µg/mL (100 µL/well) can bind Recombinant Mouse TLR6 Fc Chimera with a linear range of 0.003-2 µg/mL.

Formulation, Preparation and Storage

1533-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR6

The Toll‑like family of molecules serve as pattern recognition receptors that activate the immune system in response to microbial pathogens. Toll‑like receptors (TLRs) are type I transmembrane proteins in either the plasma membrane or the membranes of endosomal structures. TLRs contain a large number of leucine-rich repeats (LRRs) in their extracellular domains (ECD) and a cytoplasmic tail with one signal transducing Toll/IL‑1 receptor (TIR) domain (1, 2). Mature mouse TLR6 (approximately 115 kDa) consists of a 557 amino acid (aa) ECD, a 21 aa transmembrane segment, and a 190 aa cytoplasmic domain (3). Within the ECD, mouse TLR6 shares 73% and 86% aa sequence identity with human and rat TLR6, respectively. TLR6 is expressed on the surface of macrophages, monocytes, neutrophils, dermal endothelial cells, and alveolar epithelial cells in ligand‑independent association with TLR2 (4‑7). TLR2 also associates with TLR1, creating a functional receptor complex with specificity for related microbial ligands (4, 8, 9). TLR6 and TLR2 cooperate in the recognition of acylated bacterial and mycoplasma lipopeptides, peptidoglycans, and glycosylphosphatidylinositols (4‑6, 10‑13). TLR6 plays an important role in the control of Th17 responses and IL‑23 production during allergic inflammation and fungal‑induced asthma (7).

References

  1. Mills, K.H. (2011) Nat. Rev. Immunol. 11:807.
  2. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  3. Takeuchi, O. et al. (1999) Gene 231:59.
  4. Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:13766.
  5. Nakao, Y. et al. (2005) J. Immunol. 174:1566.
  6. Bulut, Y. et al. (2001) J. Immunol. 167:987.
  7. Moreira, A.P. et al. (2011) J. Clin. Invest. 121:4420.
  8. Hajjar, A.M. et al. (2001) J. Immunol. 166:15.
  9. Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
  10. Takeuchi, O. et al. (2001) Int. Immunol. 13:933.
  11. Morr, M. et al. (2002) Eur. J. Immunol. 32:3337.
  12. Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
  13. Omueti, K.O. et al. (2005) J. Biol. Chem. 280:36616.

Long Name

Toll-like Receptor 6

Alternate Names

CD286

Entrez Gene IDs

10333 (Human); 21899 (Mouse); 305353 (Rat)

Gene Symbol

TLR6

UniProt

BAA78632

Product Documents for Recombinant Mouse TLR6 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Mouse TLR6 Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

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