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Recombinant Mouse ST6GALNAC2 Protein, CF

Catalog # 6468-GT | R&D Systems, Inc. a Bio-Techne Brand
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6468-GT-020

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse ST6 Sialyltransferase 2/ST6GALNAC2 protein
Ser28-Arg373, with a C-terminal 6‑His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser28

Predicted Molecular Mass

40 kDa

SDS-PAGE

40-50 kDa, reducing conditions

Activity

Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to fetuin of fetal calf serum.
The specific activity is >700 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6468-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: ST6 Sialyltransferase 2/ST6GALNAC2

Sialic acid molecules attached to glycoproteins or glycosphingolipids play important roles in various biological processes such as immune recognition, pathogen infection, and cell adhesion. Sialyltransferases are key enzymes that regulate the serum levels of sialic acid‑containing molecules. Alpha‑N‑acetylgalactosaminide alpha‑2,6‑sialyltransferase 2, encoded by the ST6GALNAC2 gene, is a type II membrane protein localized in the Golgi network and catalyzes 2,6‑sialylation of the Tn antigen (GalNAc‑O‑Ser/Thr) (1). Sialyl‑Tn antigen is highly expressed in several human carcinomas and is associated with carcinoma aggressiveness and poor prognosis. Cells transfected with ST6GALNAC2 synthesized the sialyl‑6T structure [Gal beta1‑3 (Neu5Ac alpha2‑6)GalNAc‑O‑Ser/Thr] (2). In vitro analysis revealed that the enzyme can use both Gal beta1‑3GalNAc and Neu5Ac alpha2‑3Gal beta1-3GalNAc  as acceptor substrates (3). Recently, it has been reported that disregulation of this gene is involved in the pathogenesis of IgA nephropathy, a common kidney disease that occurs when antibody IgA accumulates in kidneys (4). The enzyme may also be used to synthesize a cancer vaccine (5, 6). At the protein level, mouse ST6GALNAC2 shows 75% amino acid sequence identity with the human homologue. The activity of this enzyme has been measured using a phosphatase‑coupled assay (7).

References

  1. Kono, M., et al. (2000) Biochem. Biophys. Res. Commun. 272:94.
  2. Marcos, N.T., et al. (2004) Cancer Res. 64:7050.
  3. Samyn-Petit, B., et al. (2000) Biochim. Biophys. Acta 1474:201.
  4. Li, G.S., et al. (2007) Hum. Mutat. 28:950.
  5. Julien, S., et al. (2009) Br. J. Cancer 100:1746.
  6. Sewell, R., et al. (2006) J. Biol. Chem. 281:3586.
  7. Wu, Z.L., et al. (2011) Glycobiology 21:727.

Long Name

ST6 alpha-N-acetylGalactosaminide alpha-2,6-sialyltransferase 2

Alternate Names

SIAT7B, SIATL1, ST6GALNAC2, STHM

Entrez Gene IDs

10610 (Human)

Gene Symbol

ST6GALNAC2

UniProt

Product Documents for Recombinant Mouse ST6GALNAC2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse ST6GALNAC2 Protein, CF

For research use only

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