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Recombinant Mouse Spinesin Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1928-SE

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1928-SE-010

Key Product Details

Source

NS0

Accession #

Structure / Form

Pro form

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Spinesin protein
Tyr61-Arg445, with an N-terminal 7-His tag
Accession # NP_109634

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

43 kDa

SDS-PAGE

55-61 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC (Catalog # ES014).
The specific activity is >1,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

1928-SE
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Spinesin

Spinesin, encoded by the TMPRSS5 gene, is a new member of type II transmembrane serine proteases (TTSPs) (1). Mouse Spinesin contains the following structural domains: a short N-terminal cytoplasmic tail, a transmembrane domain, a stem region and a serine protease domain (2). The domain structure of Spinesin is common to other TTSPs, many of which have additional domains. The stem region of Spinesin contains a scavenger receptor-like domain. There could be 4 types of transcripts due to alternative splicing (3). Type 4 predicts 10 extra amino acids at the N-terminus as compared to type 3. The ectodomain corresponding to type 3 (residues 61‑445) or  type 4 (residues 71‑455) was expressed and purified as a single chain pro-enzyme. By SDS‑PAGE, the pro‑enzyme migrates as multiple forms, possibly due to differential glycosylation. The pro‑enzyme can be activated by trypsin treatment. The resulting enzyme is active and its activity is measured as described above. The activated enzyme is a disulfide bond‑linked dimer.

References

  1. Shibata, K. et al. (2000) Genome Res. 10:1757.
  2. Yamaguchi, Y. et al. (2002) J. Biol. Chem. 277:6806.
  3. Watanable, Y. et al. (2004) Biochem. Biophys. Res. Commun. 324:333.

Alternate Names

TMPRSS5

Entrez Gene IDs

80975 (Human); 80893 (Mouse)

Gene Symbol

TMPRSS5

UniProt

Additional Spinesin Products

Product Documents for Recombinant Mouse Spinesin Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Spinesin Protein, CF

For research use only