Product Specifications for Recombinant Mouse SLITRK3 His-tag Protein, CF
Mouse myeloma cell line, NS0-derived mouse SLITRK3 protein Thr30-Ser655, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
94-105 kDa, reducing conditions
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng of protein on a nitrocellulose-coated microplate.
Scientific Data Examples for Recombinant Mouse SLITRK3 His-tag Protein, CF
Recombinant Mouse SLITRK3 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Mouse SLITRK3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 94-105 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, ≤ -20 °C under sterile conditions after reconstitution.
SLITRK3 (Slit and Trk-like family member 3) is an approximately 120 kDa type I transmembrane member of the SLITRK family of proteins which contain a Slit-like extracellular region and a Trk-like cytoplasmic region (1). The extracellular domain (ECD) of mature mouse SLITRK3 contains 6 leucine rich repeats (LRR), a C‑terminal LRR domain, and 6 more LRR flanked by a pair of N- and C‑terminal LRR domains (2, 3). Within the ECD, mouse SLITRK3 shares 98% and 99.5% amino acid sequence identity with human and rat SLITRK3, respectively. SLITRK3 is expressed in multiple regions of the brain, particularly the cerebral cortex and hippocampus (4). SLITRK3 can suppress neurite outgrowth and promote the formation of inhibitory presynaptic structures (3-6). It localizes post-synaptically and binds to PTP-delta and PTP-sigma on pre-synaptic inhibitory terminals (4, 5). SLITRK3 is up-regulated during the development of gastrointestinal stromal tumors (7).
Ko, J. (2012) Mol. Cells 34:335.
Aruga, J. et al. (2003) Gene 315:87.
Aruga, J. and K. Mikoshiba (2003) Mol. Cell Neurosci. 24:117.
Yim, Y.S. et al. (2013) Proc. Natl. Acad. Sci. USA 110:4057.
Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
Loh, K. H. et al. (2016) Cell 166:1295.
Wang, C.J. et al. (2015) World J. Gastroenterol. 21:8398.
SLIT and NTRK-like Family, Member 3
Entrez Gene IDs
22865 (Human); 386750 (Mouse); 310519 (Rat)
MGC138681, SLIT and NTRK-like family, member 3, SLITRK3
Citations for Recombinant Mouse SLITRK3 His-tag Protein, CF
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Product Documents for Recombinant Mouse SLITRK3 His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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