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Recombinant Mouse SLITRK3 His-tag Protein, CF

Catalog # 1639-SK | R&D Systems, Inc. a Bio-Techne Brand
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1639-SK-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse SLITRK3 protein
Thr30-Ser655, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr30

Predicted Molecular Mass

72 kDa

SDS-PAGE

94-105 kDa, reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng of protein on a nitrocellulose-coated microplate.

Scientific Data Images for Recombinant Mouse SLITRK3 His-tag Protein, CF

Recombinant Mouse SLITRK3 His-tag Protein SDS-PAGE

Recombinant Mouse SLITRK3 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse SLITRK3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 94-105 kDa.

Formulation, Preparation and Storage

1639-SK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: SLITRK3

SLITRK3 (Slit and Trk-like family member 3) is an approximately 120 kDa type I transmembrane member of the SLITRK family of proteins which contain a Slit-like extracellular region and a Trk-like cytoplasmic region (1). The extracellular domain (ECD) of mature mouse SLITRK3 contains 6 leucine rich repeats (LRR), a C‑terminal LRR domain, and 6 more LRR flanked by a pair of N- and C‑terminal LRR domains (2, 3). Within the ECD, mouse SLITRK3 shares 98% and 99.5% amino acid sequence identity with human and rat SLITRK3, respectively. SLITRK3 is expressed in multiple regions of the brain, particularly the cerebral cortex and hippocampus (4). SLITRK3 can suppress neurite outgrowth and promote the formation of inhibitory presynaptic structures (3-6). It localizes post-synaptically and binds to PTP-delta and PTP-sigma on pre-synaptic inhibitory terminals (4, 5). SLITRK3 is up-regulated during the development of gastrointestinal stromal tumors (7).

References

  1. Ko, J. (2012) Mol. Cells 34:335.
  2. Aruga, J. et al. (2003) Gene 315:87.
  3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell Neurosci. 24:117.
  4. Yim, Y.S. et al. (2013) Proc. Natl. Acad. Sci. USA 110:4057.
  5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
  6. Loh, K. H. et al. (2016) Cell 166:1295.
  7. Wang, C.J. et al. (2015) World J. Gastroenterol. 21:8398.

Long Name

SLIT and NTRK-like Family, Member 3

Alternate Names

MGC138681, SLIT and NTRK-like family, member 3

Entrez Gene IDs

22865 (Human); 386750 (Mouse); 310519 (Rat)

Gene Symbol

SLITRK3

UniProt

Product Documents for Recombinant Mouse SLITRK3 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse SLITRK3 His-tag Protein, CF

For research use only

Reconstitution Calculator

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