Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Recombinant Mouse SIRP beta 1B Fc Chimera Protein, CF

Catalog # 10335-SB | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
10335-SB-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse SIRP beta 1B protein
Mouse SIRP beta 1B
(Met28-Gly360)
Accession # NP_001166931.1
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met28 and Arg29

Predicted Molecular Mass

64 kDa

SDS-PAGE

70-95 kDa and 175-200 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse SIRP beta 1B Fc Chimera (Catalog # 10335-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SP‑D (Catalog # 1920-SP) binds with an ED50 of 40-320 ng/mL.

Scientific Data Images for Recombinant Mouse SIRP beta 1B Fc Chimera Protein, CF

Recombinant Mouse SIRP beta 1B Fc Chimera Protein Binding Activity

Recombinant Mouse SIRP beta 1B Fc Chimera Protein Binding Activity

When Recombinant Mouse SIRP beta 1B Fc Chimera (Catalog # 10335-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SP-D (Catalog # 1920-SP) binds with an ED50 of 40-320 ng/mL.

Formulation, Preparation and Storage

10335-SB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP beta 1B

Signal-regulatory protein beta 1b (SIRP beta 1b), or CD172b, is a disulfide-linked type I membrane glycoprotein belonging to the SIRP/SHPS/CD172 family of the immunoglobulin (Ig) superfamily. The three SIRP family members are paired receptors that have a conserved extracellular domain (ECD) but differing C-terminal domains and functions (1). The ECD of SIRP beta 1b contains one V-set Ig and two C1-set Ig domains. The mature ECD of mouse SIRP beta 1b shares 57% amino acid sequence identity with human SIRP beta 1. Proteins in the SIRP family are typically expressed in cells of monocyte, macrophage or dendritic lineages and positively charged residues within the transmembrane domain mediate interactions with DAP12 proteins which contain immunoreceptor tyrosine-based activation motifs (ITAMs) (3, 4). SIRP beta 1 has a relatively short cytoplasmic region and lacks the signaling motifs for association with phosphatases. However, formation of the SIRP beta 1/DAP12 complex in myeloid cells induces tyrosine phosphorylation, mitogen-activated protein kinase activation, and cellular activation (5, 6). Engagement of SIRP beta 1 by specific monoclonal antibodies promoted Fc gamma receptor-dependent or - independent phagocytosis in mouse peritoneal macrophages (7). Surfactant protein D (Sp-D) has been shown to bind SIRP alpha and SIRP beta 1 in a calcium-dependent and sugar-specific manner on a distinct binding site from CD47 (8). Although the SIRP beta 1 extracellular regions share a high degree of homology with the SIRP alpha, SIRP beta 1 has been shown not to bind CD47 (9).

References

  1. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
  2. van den Berg, T. et al. (2008) Trends in Immunology 29:203.
  3. Liu, Y. et al. (2005) Journal of Biological Chemistry 280:36132.
  4. Matozaki, T. et al. (2009) Trends in Cell Biology 19:72.
  5. Dietrich, J. et al. (2000) J. Immunol. 164:9.
  6. Brook, G. et al. (2004) J. Immunol. 173:2562.
  7. Hayashi, A. et al. (2004) J. Biol. Chem. 279:29450.
  8. Fournier B. et al. (2012) J. Biol. Chem. 287:19386.
  9. Seiffert M. et al. (2001) Blood 97:2741.

Long Name

Signal-regulatory Protein beta 1B

Entrez Gene IDs

668101 (Mouse)

Gene Symbol

SIRPB1B

UniProt

Additional SIRP beta 1B Products

Product Documents for Recombinant Mouse SIRP beta 1B Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse SIRP beta 1B Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Loading...
Setting the standard in quality research reagents