Key Product Details
Structure / Form
Val24-Arg611, with a C-terminal 6-His tag
N-terminal Sequence Analysis
Predicted Molecular Mass
5 µg/mL of rmPodocan can significantly retard the rate of type I collagen fibrillogenesis.
Formulation, Preparation and Storage
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
Reconstitute at 100 μg/mL in sterile PBS.
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse Podocan is a 95 kDa, secreted glycoprotein that is a class V member of the small leucine rich protein gene family (1, 2). It is synthesized as a 611 amino acid (aa) precursor that contains a 23 aa signal sequence, a cysteine-rich region, a series of leucine rich repeats (LRRs), and an extensive acidic C-terminal domain (4, 5). The 15 aa cysteine-rich region (aa 69 - 84) shows a CX3CXCX7C motif, qualifying it as a class V SLRP family member. This is followed by twenty LRRs, thirteen of which are type T (4xLeu; 1xPhe) and seven type S (4xLeu; 2xPro). The LRRs run uninterrupted from aa 89 ‑ 559. The C-terminal seventeen amino acids contain fourteen Glu residues. The negative charge associated with these residues may play a role in basement membrane permeability (4). Mature mouse Podocan is 93% and 94% aa identical to human and canine Podocan, respectively. Over the last 504 aa, mouse Podocan is 98% aa identical to rat Podocan. Podocan is apparently secreted by podocytes and vascular endothelial cells, and deposited in the underlying basement membrane (4). Podocan is known to bind to type I collagen, and have an inhibitory effect on the migration of Podocan-transfected CHO cells (5). The significance of this is unclear.
- Matsushima, N. et al. (2000) Proteins 38:210.
- Matsushima, N. et al. (2004) Proteins 54:394.
- Ross, M.D. et al. (2005) Cell. Mol. Life Sci. 62:2771.
- Ross, M.D. et al. (2003) J. Biol. Chem. 278:33248.
- Shimizu-Hirota, R. et al. (2004) FEBS Lett. 563:69.
Product Specific Notices for Recombinant Mouse Podocan Protein, CF
For research use only