Mouse myeloma cell line, NS0-derived mouse Pappalysin-1/PAPP-A protein Ala81-Gly1624 with a C-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
141-200 kDa, under reducing conditions
Measured by its ability to cleave IGFBP-5.
Recombinant Mouse Pappalysin-1/PAPPA will cleave >50% of Recombinant Human IGFBP‑5 (Catalog # 875-B5), as measured under the described conditions.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris, CaCl2, NaCl and CHAPS.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serralysins (1). Pappalysin-1 (PAPP-A) is a secreted, disulfide-linked 400 kDa homodimer with an N-terminal catalytic domain and five C-terminal Sushi domains (2). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (3). PAPP-A also contains three Lin12-Notch repeats (LNR) that bind calcium and are required for cleavage of IGFBP-4, but not IGFBP-5 (2). PAPP-A is thought to be the only physiological IGFBP-4 proteinase supported by the observation that no IGFBP-4 cleavage occurred in embryonic fibroblasts derived from PAPP-A knockout mice (4). As a regulator of IGF bioavailability, and consequently IGF signaling, PAPP-A is an attractive therapeutic target (5-8). PAPP-A knockout mice have an extended lifespan (9) and use of specific neutralizing antibodies causes inhibition of atherosclerotic plaque progression (10). PAPP-A levels are elevated in cancer (11-14) and PAPP-A has been identified as a migration/invasion-promoting gene (5,6, 12). PAPP-A is also important during pregnancy where PAPP-A levels are increased in the plasma by a factor of about 150. Low maternal serum levels of PAPP-A is used as an indication of Down syndrome or other fetal aneuploidies in the first trimester of pregnancy (15).
Boldt, H. B. et al. (2001) Biochem. J. 358:359.
Boldt, H. B. et al. (2004) J. Biol. Chem. 279:38525.
Laursen, L. S. et al. (2001) FEBS Lett. 504:36.
Conover, C. A. et al. (2004) Development 131:1187.
Huang, J. et al. (2013) Oncotarget. 4:1172.
Prithviraj, P. et al. (2015) Oncotarget 6: 15953.
Yu, X. E. et al. (2018) Atherosclerosis. 278:250.
Guo, Y. et al. (2018) Am. J. Cancer Res. 8:955.
Conover, C. A. et al. (2010) J. Gerontol. A. Biol. Sci. Med. Sci. 65:590.
Conover, C. A. et al. (2016) J Cardiovasc. Transl. Res. 9:77.
Bulut, I. et al. (2009) Am. J. Med. Sci. 337: 241.
Boldt, H. B. and Conover C. A. (2011) Endocrinology 152:1470.
Pregnancy-associated Plasma Protein 1
Entrez Gene IDs
5069 (Human); 18491 (Mouse)
ASBABP2, DIPLA1, EC 220.127.116.11, IGF-dependent IGFBP-4 protease, IGFBP-4ase, IGFBP-4aseaspecific BCL2 ARE-binding protein 2, Insulin-like growth factor-dependent IGF-binding protein 4 protease, PAPA, PAPP-A1, PAPP-Adifferentially placenta 1 expressed protein, PAPPA, PAPPA1, Pappalysin-1, Pappalysin1, Pregnancy-associated plasma protein A, insulin-like growth factor-dependent IGF binding protein-4 protease, pregnacy-associated plasma protein A, pregnancy-associated plasma protein A, pappalysin 1
Citations for Recombinant Mouse Pappalysin-1/PAPP-A His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
Setting the standard in quality research reagents for over 30 years
A trusted leader in quality life science reagents
Your trusted supplier for innovative and high performance life science reagents
Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis
Proprietary RNAscope® technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity
A world leader in developing liquid biopsy based diagnostics