Source	Mouse myeloma cell line, NS0-derived mouse Pappalysin-1/PAPP-A protein Ala81-Gly1624 with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala81
Predicted Molecular Mass	173 kDa
SDS-PAGE	141-200 kDa, under reducing conditions
Activity	Measured by its ability to cleave IGFBP-5. Recombinant Mouse Pappalysin-1/PAPPA will cleave >50% of Recombinant Human IGFBP‑5 (Catalog # 875-B5), as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10174-ZN

Formulation	Supplied as a 0.2 μm filtered solution in Tris, CaCl₂, NaCl and CHAPS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: Pappalysin-1/PAPP-A

Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serralysins (1). Pappalysin-1 (PAPP-A) is a secreted, disulfide-linked 400 kDa homodimer with an N-terminal catalytic domain and five C-terminal Sushi domains (2). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (3). PAPP-A also contains three Lin12-Notch repeats (LNR) that bind calcium and are required for cleavage of IGFBP-4, but not IGFBP-5 (2). PAPP-A is thought to be the only physiological IGFBP-4 proteinase supported by the observation that no IGFBP-4 cleavage occurred in embryonic fibroblasts derived from PAPP-A knockout mice (4). As a regulator of IGF bioavailability, and consequently IGF signaling, PAPP-A is an attractive therapeutic target (5-8). PAPP-A knockout mice have an extended lifespan (9) and use of specific neutralizing antibodies causes inhibition of atherosclerotic plaque progression (10). PAPP-A levels are elevated in cancer (11-14) and PAPP-A has been identified as a migration/invasion-promoting gene (5,6, 12). PAPP-A is also important during pregnancy where PAPP-A levels are increased in the plasma by a factor of about 150. Low maternal serum levels of PAPP-A is used as an indication of Down syndrome or other fetal aneuploidies in the first trimester of pregnancy (15).

Item	Value
References	Boldt, H. B. et al. (2001) Biochem. J. 358:359. Boldt, H. B. et al. (2004) J. Biol. Chem. 279:38525. Laursen, L. S. et al. (2001) FEBS Lett. 504:36. Conover, C. A. et al. (2004) Development 131:1187. Huang, J. et al. (2013) Oncotarget. 4:1172. Prithviraj, P. et al. (2015) Oncotarget 6: 15953. Yu, X. E. et al. (2018) Atherosclerosis. 278:250. Guo, Y. et al. (2018) Am. J. Cancer Res. 8:955. Conover, C. A. et al. (2010) J. Gerontol. A. Biol. Sci. Med. Sci. 65:590. Conover, C. A. et al. (2016) J Cardiovasc. Transl. Res. 9:77. Bulut, I. et al. (2009) Am. J. Med. Sci. 337: 241. Boldt, H. B. and Conover C. A. (2011) Endocrinology 152:1470.
Long Name	Pregnancy-associated Plasma Protein 1
Entrez Gene IDs	5069 (Human); 18491 (Mouse)
Alternate Names	ASBABP2, DIPLA1, EC 3.4.24.79, IGF-dependent IGFBP-4 protease, IGFBP-4ase, IGFBP-4aseaspecific BCL2 ARE-binding protein 2, Insulin-like growth factor-dependent IGF-binding protein 4 protease, PAPA, PAPP-A1, PAPP-Adifferentially placenta 1 expressed protein, PAPPA, PAPPA1, Pappalysin-1, Pappalysin1, Pregnancy-associated plasma protein A, insulin-like growth factor-dependent IGF binding protein-4 protease, pregnacy-associated plasma protein A, pregnancy-associated plasma protein A, pappalysin 1

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Recombinant Mouse Pappalysin-1/PAPP-A His-tag Protein, CF

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Background: Pappalysin-1/PAPP-A

Citations for Recombinant Mouse Pappalysin-1/PAPP-A His-tag Protein, CF

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