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Recombinant Mouse Olfactomedin-1/Noelin-1 Protein, CF

Catalog # 4636-NL | R&D Systems, Inc. a Bio-Techne Brand
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4636-NL-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Olfactomedin-1/Noelin-1 protein
Ser2-Arg481 , with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser2

Predicted Molecular Mass

55.7 kDa

SDS-PAGE

80-90 kDa, reducing conditions

Activity

Measured by its ability to induce epithelial to mesenchymal transformation of MDCKII canine kidney epithelial cells.
Treatment of MDCKII cells with 200 ng/mL of rmNoelin-1 for 3 days is sufficient to induce the transformation of MDCKII cells.

Formulation, Preparation and Storage

4636-NL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Olfactomedin-1/Noelin-1

Noelin-1 (neuronal olfactomedin-related endoplasmic reticulum-localized-1), also known as Olfactomedin-1 or pancortin, is a 75 kDa secreted extracellular matrix glycoprotein expressed in the brain (1 - 3). Alternate promoter usage and splicing creates four forms of Noelin (Noelin 1 - 4) that are combinations of a common central region with alternate N- and C-termini (3, 4). Noelin-1 is the longest of the four isoforms, encoding a 16 amino acid (aa) signal sequence and a 469 aa mature protein with a coiled-coil region for multimerization and an olfactomedin-like domain. The shortest isoforms, Noelin-3 and -4, are truncated within the coiled-coil region and lack the olfactomedin-like domain (4). In the species tested, these isoforms appear to oppose actions of the longer isoforms (5). All isoforms are capable of forming homo- or heterodimers through cysteines in their common central region (2). The C-terminus of Noelin-1 (mouse aa 482 - 485) contains a putative endoplasmic reticulum retention sequence that has not been included in the R&D Systems protein (6). Mouse Noelin-1 (aa 17 - 481) shares > 99%, 99%, 97%, 96% and 93% aa identity with corresponding regions of rat, human, opossum, chicken and Xenopus Noelin-1, respectively. In vivo expression patterns differ somewhat between species (1). In chick early development, Noelin-1 shows highest concentration in areas that produce neural crest cells and is produced by migrating neural crest cells (7). Overproduction of Noelin-1 prolongs neural crest cell formation (7). Noelin-1 is also expressed in mouse neural crest, but not frog (1 - 3). All three species express Noelin-1 later in differentiating neural tissues in the spinal cord, brain and cranial ganglia (1 - 3).

References

  1. Moreno, T.A. & M. Bronner-Fraser (2002) Mech. Dev. 119:121.
  2. Ando, K. et al. (2005) Neuroscience 133:947.
  3. Nagano, T. et al. (1998) Brain Res. Mol. Brain Res. 53:13.
  4. Danielson, P.E. et al. (1994) J. Neurosci. Res. 38:468.
  5. Moreno, T.A. & M. Bronner-Fraser (2005) Dev. Biol. 288:434.
  6. Moreno, T.A. & M. Bronner-Fraser (2001) Dev. Biol. 240:340.
  7. Barembaum, M. et al. (2000) Nat. Cell Biol. 2:219.

Alternate Names

AMY, NOE1, NOEL1, Noelin-1, OlfA, Olfactomedin1, OLFM1

Entrez Gene IDs

10439 (Human)

Gene Symbol

OLFM1

UniProt

Product Documents for Recombinant Mouse Olfactomedin-1/Noelin-1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Olfactomedin-1/Noelin-1 Protein, CF

For research use only

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