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Recombinant Mouse OCAM/NCAM2 His-tag Protein, CF

Catalog # 10154-CM | R&D Systems, Inc. a Bio-Techne Brand
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10154-CM-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse OCAM/NCAM2 protein
Met1-Asn697, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu20

Predicted Molecular Mass

76.8 kDa

SDS-PAGE

95-110 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of T47D human breast cancer cells.
The ED50 for this effect is 0.6-3.6 μg/mL.

Scientific Data Images for Recombinant Mouse OCAM/NCAM2 His-tag Protein, CF

Recombinant Mouse OCAM/NCAM2 His-tag Protein Bioactivity

Recombinant Mouse OCAM/NCAM2 His-tag Protein Bioactivity

Immobilized Recombinant Mouse OCAM/NCAM2 His-tag (Catalog # 10154-CM) supports the adhesion of T47D human breast cancer cells. The ED50 for this effect is 0.6-3.6 μg/mL.
Recombinant Mouse OCAM/NCAM2 His-tag Protein SDS-PAGE

Recombinant Mouse OCAM/NCAM2 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse OCAM/NCAM2 His-tag (Catalog # 10154-CM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-110 kDa.

Formulation, Preparation and Storage

10154-CM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OCAM/NCAM2

OCAM (olfactory cell adhesion molecule), also known as NCAM2 (neural cell adhesion molecule 2) and RNCAM (Rb‑8 neural cell adhesion molecule) is an approximately 100 kDa type I transmembrane glycoprotein belonging to the NCAM family within the immunoglobulin superfamily (1‑5). OCAM dimerizes and participates in homophilic adhesion, but unlike NCAM1/CD56, it is not polysialic acid‑glycosylated, and does not participate in heparin sulfate binding or heterophilic adhesion (2). The 837 amino acid (aa) form of human or mouse OCAM contains a 19 aa signal sequence, a 678 aa extracellular domain (ECD) with five Ig‑like C2‑type domains and two fibronectin type III domains, a transmembrane domain and a 119 aa cytoplasmic domain (2‑4). The first two Ig‑like domains mediate homodimer formation in trans (6). In mouse, a glycosyl phosphatidylinositol‑linked form may show differential expression and function on olfactory axons (1‑3, 7). The ECD of mouse OCAM shares 93%, 98% and 92% aa sequence identity with human, rat, and bovine OCAM, respectively. OCAM is expressed by a subset of axons in the olfactory, vomeronasal, and retrosplenial cortex in a zone‑specific manner (1‑3, 7‑9). It is thought to be important for organization of axons and dendrites and segregation of axodendritic and dendrodendritic synapses within glomeruli (1, 7, 10, 11). Because OCAM is encoded on chromosome 21 in humans, it is implicated in neurological abnormalities in Down Syndrome (trisomy 21) (1, 3, 4). It may also be implicated in development of autism and Alzheimer's disease, and over‑expressed in some breast and prostate cancers (1, 5).

References

  1. Winther, M. et al. (2012) Int. J. Biochem. Cell Biol. 44:441.
  2. Yoshihara, Y. et al. (1997) J. Neurosci. 17:5930.
  3. Alenius, M. and S. Bohm (1997) J. Biol. Chem. 272:26083.
  4. Paoloni-Giacobino, A. et al. (1997) Genomics 43:43.
  5. Takahashi, S. et al. (2011) Cancer Sci. 102:808.
  6. Kulahin, N. et al. (2011) Structure 19:203.
  7. Alenius, M. and S. Bohm (2003) Development 130:917.
  8. von Campenhausen, H. et al. (1997) Neuroreport 8:2607.
  9. Ichinohe, N. et al. (2003) Eur. J. Neurosci. 18:1764.
  10. Walz, A. et al. (2006) Mol. Cell. Neurosci. 32:1.
  11. Borisovska, M. et al. (2011) J. Physiol. 589:1927.

Long Name

Opioid-binding and Cell Adhesion Molecule/Neural Cell Adhesion Molecule 2

Alternate Names

NCAM2, Rb-8, RNCAM

Entrez Gene IDs

4685 (Human); 17968 (Mouse)

Gene Symbol

NCAM2

UniProt

Product Documents for Recombinant Mouse OCAM/NCAM2 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse OCAM/NCAM2 His-tag Protein, CF

For research use only

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