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Recombinant Mouse Netrin-G2a Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 2744-NG

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2744-NG-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Netrin-G2a protein
Mouse Netrin-G2a
(Asp18 - Arg504)
Accession # NP_598007
IEGRMDP Mouse IgG2A
(Glu98 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp18

Predicted Molecular Mass

82.5 kDa (monomer)

SDS-PAGE

100-105 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When recombinant human LRRC4 (Catalog # 4995-LR) is coated at 5 μg/mL, recombinant mouse Netrin-G2a/Fc Chimera binds with an apparent K

Formulation, Preparation and Storage

2744-NG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 400 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Netrin-G2a

Netrin-G2a (netr: Sanskrit for "one who guides") is a 60 ‑ 75 kDa glycoprotein member of the netrin‑G/laminet subfamily, UNC-6/netrin family of axon guidance molecules (1, 2, 3). The two members of the netrin‑G subfamily (G1 and G2) are GPI‑linked and found only in vertebrates. They do not bind to either DCC or UNC5, but regulate synaptic interactions by binding to NGL-1 and NGL-2. Mouse Netrin-G2a (also called laminet-2 and NTNG2) is the shortest of three netrin‑G2 gene products (2). It is synthesized as a 530 amino acid (aa) preprocursor that contains a 17 aa signal sequence, a 490 aa mature region, and a 23 aa C‑terminal prosegment that is cleaved to give rise to a GPI‑linkage site (Gly507). The mature molecule contains one N‑terminal laminin type VI domain (aa 60 ‑ 285), plus three consecutive laminin EGF‑like regions (aa 287 ‑ 453). There are at least two splice variants, each of which show insertions after Gly352. The first (netrin‑G2b) shows a 60 aa substitution for Asn353, while the second (netrin‑G2c) shows a 35 aa substitution for Asn353. Mature mouse Netrin‑G2a shares 99% and 96% aa identity with rat and human Netrin‑G2a, respectively. Netrin‑G2a is principally expressed in neurons, including granule cells of the dentate gyrus, pyramidal cells of the hippocampus (CA1, CA3, CA4) and cells of the cerebral cortex (4, 5, 6). It appears to be a presynaptic molecule that is associated with excitatory intracortical tracts (6 ‑ 9). The ligand for Netrin‑G2a is NGL‑2/netrin‑G ligand #2, a postsynaptic 100 kDa member of the NGL family of synaptic cell adhesion molecules (8, 10). Netrin‑G2a:NGL‑2 binding is of high affinity (K= 2.5 nM), and appears to influence the stability of NMDA‑type glutamatergic receptors on postsynaptic membranes (4, 11). Although the exact mechanism is unclear, Netrin‑G2a ligation of NGL‑2 promotes NGL-2 interaction with PSD95 and NR1 and NR2B in the postsynaptic membrane. This leads to an association of both neuroligin‑1/2 and NMDA subunits, and the formation of functional, fixed glutamatergic receptors (5, 6, 8).

References

  1. Yin, Y. et al. (2002) Mol. Cell. Neurosci. 19:344.
  2. Nakashiba, T. et al. (2002) Mech. Dev. 111:47.
  3. Meerabux, J. et al. (2005) Genomics 86:112.
  4. Eastwood, S.L. & P.J. Harrison (2008) Neuropsychpharmacology 33:933.
  5. Pan, Y. et al. (2010) Exp. Neurol. Apr. 9 [Epub ahead of print].
  6. Nishimura-Akiyoshi, S. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14801.
  7. Miyashita, T. et al. (2005) Neuroscience 136:487.
  8. Kim, S. et al. (2006) Nat. Neurosci. 9:1294.
  9. Niimi, K. et al. (2007) J. Neuroimmunol. 192:99.
  10. Woo, J. et al. (2009) Mol. Cell. Neurosci. 42:1.
  11. Zhang, W. et al. (2008) Genes Brain Behav. 7:385.

Alternate Names

Lmnt2a, NetrinG2a, NTG2a

Entrez Gene IDs

84628 (Human); 171171 (Mouse)

Gene Symbol

NTNG2

UniProt

Additional Netrin-G2a Products

Product Documents for Recombinant Mouse Netrin-G2a Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Netrin-G2a Fc Chimera Protein, CF

For research use only

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