Recombinant Mouse Netrin-4 Protein

Name: Recombinant Mouse Netrin-4 Protein
Brand: R&D Systems
SKU: 1132-N4

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1132-N4

Citations (4)

Product Datasheet / COA / SDS

Discontinued Product

1132-N4 has been discontinued. View all Netrin-4 products.

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	Q9JI33
Applications:	Binding Activity

View Full Technical Details for Recombinant Mouse Netrin-4 Protein

Product Specifications for Recombinant Mouse Netrin-4 Protein

Source	Mouse myeloma cell line, NS0-derived mouse Netrin-4 protein Leu19-Val628 & Ala26-Val628, both with a C-terminal 10-His tag
Purity	>80%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.1 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu19 & Ala26
Predicted Molecular Mass	69 kDa
SDS-PAGE	85 kDa, reducing conditions
Activity	Measured by its ability to bind rrUNC5H2/Fc Chimera in a functional ELISA. Immobilized rrUNC5H2/Fc Chimera at 5 µg/mL (100 µL/well) can bind rmNetrin-4 with a linear range of 6-400 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1132-N4

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Netrin-4

Netrins/UNC-6 (netr: Sanskrit for “one who guides”) are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins binds to the DCC and UNC5 family of receptors to attract or repel axons. Mouse Netrin-4, also known as beta -Netrin, is synthesized as a 629 amino acid (aa) precursor that contains a 19 aa signal sequence, a 428 aalaminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a 182 aa C domain rich in basic aa residues that serves as a heparin binding site. Netrin-4 has been reported to exist as both a monomer and a dimer. The dimeric form was reported to be the less active of the two. Mouse Netrin-4 shares 31%, 29% and 25% aa sequence identity with mouse Netrin-1, -3, and -G1a, respectively. It also shares 89% aa sequence identity with human Netrin-4. Netrin-4 has widespread expression, occurring in nervous tissues such as embryonic floor plate and postnatal neurons such as cerebellar granule cells and hippocampal pyramidal cells. It also is found in non-neural tissues such as adult Bowman’s capsule and medullary tubular epithelium in kidney, and embryonic pancreatic and intestinal epithelium, plus cells of the ureteric bud. Netrin-4 expression is often associated with basement membrane. Netrin-4 has been shown to initiate axon outgrowth from olfactory bulb explants (1 - 7).

Item	Value
References	McFarlane, S. (2000) Biochem. Cell. Biol. 78:563. Kennedy, T.E. (2000) Biochem. Cell Biol. 78:569. Yu, T.W. and C.I. Bargmann (2001) Nature Neurosci. 4(Suppl):1169. Stein, E. et al. (2001) Science 291:1976. Koch, M. et al. (2000) J. Cell Biol. 151:221. Yin, Y. et al. (2000) Mech. Dev. 96:115. Livesey, F.J. (1999) Cell. Mol. Life Sci. 56:62.
Entrez Gene IDs	59277 (Human); 57764 (Mouse)
Alternate Names	NTN4, Netrin-4, Netrin4, PRO3091, beta-Netrin, hepar-derived netrin-like protein, netrin 4

Product Specific Notices for Recombinant Mouse Netrin-4 Protein

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.

Citations for Recombinant Mouse Netrin-4 Protein (4)

Showing 1 - 4 of 4 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Netrin-4 Protein include:

Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Toshihide Yamashita (2016), Dorsal horn interneuron-derived Netrin-4 contributes to spinal sensitization in chronic pain via Unc5B J. Exp. Med., 0(0).
PMID: 27856613
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Han et al. (2015), Therapeutic effects of topical netrin-4 inhibits corneal neovascularization in alkali-burn rats. PLoS ONE, 10(4):e0122951.
PMID: 25853509
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Hoang S et al. (2009), Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia. J. Cereb. Blood Flow Metab., 29(2):385-97.
PMID: 18985053
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Wang W et al. (2008), Netrin-1 and kidney injury. I. Netrin-1 protects against ischemia-reperfusion injury of the kidney. Am. J. Physiol. Renal Physiol., 294(4):F739-47.
PMID: 18216145

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Showing 1 - 4 of 4 citations Showing All

Product Documents for Recombinant Mouse Netrin-4 Protein

Product Datasheets

Download Product Datasheets: 1132-N4 (with carrier)

COA

SDS

Download SDS 1132-N4 (with carrier)

Download SDS 1132-N4/CF (carrier free)