Recombinant Mouse Mimecan Protein, CF

Name: Recombinant Mouse Mimecan Protein, CF
Brand: R&D Systems
SKU: 2949-MC
Price: 417 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 2949-MC

Citations (5)

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Key Product Details

Source:	NS0
Accession #:	Q62000
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse Mimecan Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse Mimecan protein Ala20-Phe298, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala20
Predicted Molecular Mass	32.6 kDa
SDS-PAGE	50 kDa, reducing conditions
Activity	Measured by its ability to modulate collagen fibrillogenesis. Ge, G. et al. (2004) J. Biol. Chem. 279:41626. At 15 µg/mL, rmMimecan can significantly retard the rate of type I collagen fibrillogenesis.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2949-MC

Formulation	Supplied as a 0.2 μm filtered solution in PBS.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening.

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Background: Mimecan

Mimecan is a 34 kDa secreted monomeric glycoprotein that belongs to the SLRP, or small leucine-rich proteoglycan family of matrix molecules (1, 2). All SLRP family members contain multiple leucine rich repeats, of which there are two types; a 21 amino acid (aa) proline-containing S-type, and a 26 aa phenylalanine-containing T-type (2, 3). Mimecan is a class III SLRP subfamily member, which means it contains an S-T (vs. S-T-T) repeating motif. Mouse Mimecan is synthesized as a 298 aa precursor that contains a 19 aa signal sequence and a 279 aa mature region (3, 4). The mature region contains a 75 aa N-terminus followed by a 13 aa cysteine-rich region and seven consecutive LRR repeats. There is one (presumably occupied) C-terminal N-linked glycosylation site and a potential keratan sulfate attachment point in LRR #4 (aa 161 - 180) (3). Nomenclature for the mimecan molecule is confusing. Mimecan is best thought of as being the full-length, 279 aa glycoprotein (5). Proteolytic removal of the first 56 aa by BMP-1/Tolloid-like proteinases generates the 25 kDa KSPG form (aa 57 - 279) (3). The 17 kDa, 56 aa mature N-terminus has been referred to as the minecam prosegment (3). When the prosegment is removed, mature mouse KSPG (aa 57 - 279) is 98%, 91%, 90% and 91% aa identical to rat, bovine, human and canine KSPG, respectively. A 12 kDa form (aa 175 - 279) consisting of the 105 aa of the C-terminus (OIF/osteoglycan) has also been observed (6). Noncoding alternate splice forms for mouse are believed to exist and should be expressed in a tissue specific fashion (7, 8). No splice forms are known that impact the coding region. Mimecan seems to exist as proteoglycan (PG) and non-PG forms. In cornea, keratan sulfate is present, although the exact function of the PG is unknown. The corneal extracellular matrix is normally transparent. While mimecan is reported to inhibit fibrillogenesis, possibly by limiting lateral accretion of collagen monomers, it is unclear if the mimican PG actually contributes to transparency (3, 9). In other tissues, mimecan and its derivatives can exist as simple glycoproteins, absent of any PG adduct (6). The uncertain nature of mimecan’s structure and function gives rise to its name: Mime is a Norse-legend dwarf that was notoriously deceitful and misleading (6).

Item	Value
References	Iozzo, R.V. (1999) J. Biol. Chem. 275:18843. Matsushima, N. et al. (2000) Proteins 38:210. Ge, G. et al. (2004) J. Biol. Chem. 279:41626. Ujita, M. et al. (1995) Gene 158:237. Hu, S-M. et al. (2005) J. Clin. Endocrinol. Metab. 90:6657 Funderburgh, J.L. et al. (1997) J. Biol. Chem. 272:28089. Tasheva, E.S. et al. (1999) J. Biol. Chem. 274:18693. Tasheva, E.S. et al. (1997) J. Biol. Chem. 272:32551. Tasheva, E.S. et al. (2002) Mol. Vis. 8:407.
Entrez Gene IDs	4969 (Human); 18295 (Mouse)
Alternate Names	DKFZp586P2421, KSPG25, Mimecan, OGN, OIF, OIFOG, Osteoinductive factor, SLRR3A, SLRR3Acorneal keratan sulfate proteoglycan, mimecan proteoglycan, osteoglycin, osteoglycin (osteoinductive factor)

Citations for Recombinant Mouse Mimecan Protein, CF (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Mimecan Protein, CF include:

Species: Snake
Sample Types: Protein
Applications: Bioassay

AF Asega et al. (2020), Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases Sci Rep, 10(1):12912.
PMID: 32737331
Species: Mouse
Sample Types: Recombinant Protein
Applications: Bioassay

AF Asega et al. (2020), Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases Sci Rep, 10(1):12912.
PMID: 32737331
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

NJ Lee et al. (2018), Osteoglycin, a novel coordinator of bone and glucose homeostasis Mol Metab, 13(0):30-44.
PMID: 29799418
Species: Mouse
Sample Types: Protein
Applications: Enzyme Assay

Zhang Y et al. (2013), Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases. Invest Ophthalmol Vis Sci, 54(2):1014-25.
PMID: 23322569
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay

Zhang Y et al. (2011), Effects of Ultraviolet-A and Riboflavin on the Interaction of Collagen and Proteoglycans during Corneal Cross-linking. J. Biol. Chem., 286(15):13011-22.
PMID: 21335557

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Showing 1 - 5 of 5 citations Showing All

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