Recombinant Mouse Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF

Name: Recombinant Mouse Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF
Brand: R&D Systems
SKU: 1008-LR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1008-LR

Citations (2)

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	P50284
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF

Source

Mouse myeloma cell line, NS0-derived mouse Lymphotoxin beta R/TNFRSF3 protein

Mouse Lymphotoxin beta R (Ser28-Pro218) Accession # P50284	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser28

Predicted Molecular Mass

48.7 kDa (monomer)

SDS-PAGE

63-67 kDa, reducing conditions

Activity

Measured by its ability to inhibit Lymphotoxin alpha 1/ beta 2-induced IL-8 secretion in A375 human melanoma cells. Degli-Esposti, M. et al. (1997) J. Immunol. 158:1756. The ED₅₀ for this effect is 15-75 ng/mL in the presence of 10 ng/mL of Recombinant Human Lymphotoxin alpha 1/ beta 2 (Catalog #
8884-LY).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1008-LR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin beta R (LT beta R), previously called TNF RIII or TNF R‑related protein (TNF Rrp), is a type I transmembrane glycoprotein within the TNF receptor superfamily, designated TNFRSF3 (1, 2). Mouse LT beta R cDNA encodes 415 amino acids (aa) including a 30 aa signal peptide, a 193 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 171 aa cytoplasmic domain. The ECD contains four cysteine‑rich motifs characteristic of the TNF receptor superfamily (1). Within the ECD, mouse LT beta R shares aa sequence identity of 91% aa with rat, and 65-71% with human, canine, porcine, equine and bovine LT beta R. Soluble LT beta R can be formed by proteolytic cleavage of the ECD, and is an inhibitor of transmembrane LT beta R, as is recombinant LT beta R, which inhibits autoimmunity (2-5). A potential mouse isoform that ends at aa 218, just prior to the transmembrane domain, could also be secreted and inhibitory (6). LT beta R is expressed by visceral, lymphoid, and other stroma, epithelia and myeloid cells, but not lymphocytes (1, 3). LT beta R ligands include homotrimers of LIGHT (TNFSF14; also a ligand for HVEM) and the heterotrimeric lymphotoxin LT alpha 1/ beta 2 (2, 3, 5). Depending on the cell type and expression of TRAF3, activation of LT beta R has been shown to induce canonical (IKK/RelA; pro‑inflammatory) or alternative (NIK/RelB; lymphoid organogenic) NF kappa B activation (5, 7). LT beta R is expressed on mesenchymal stromal organizing cells that give rise to stroma of primary (thymus), secondary (tonsils, lymph nodes and Peyers patches) and tertiary (ectopic inflammatory) lymphoid structures (2-4, 8-10). Secondary immune tissues are absent in LT beta R‑deficient mice (2-4). LT beta R engagement induces production of IL‑7, RANK, TRANCE/RANK L, VEGF‑C, adhesion molecules such as VCAM‑1, ICAM‑1 and MAdCAM, and chemokines such as CXCL13, CCL19 and CCL21 (2, 8-10). LT beta R is expressed by hepatocytes, is up-regulated in regeneration, hepatitis and hepatocellular carcinoma, and influences lipid metabolism and atherosclerosis (3, 5, 11). It regulates cell growth and can initiate inflammation‑related carcinogenesis (5, 11).

Item	Value
References	Force, W.R. et al. (1995) J. Immunol. 155:5280. McCarthy, D.D. (2006) Immunol. Res. 35:41. Tumanov, A.V. et al. (2007) Curr. Mol. Med. 7:567. Boehm, T. et al. (2003) J. Exp. Med. 198:757. Wolf, M.J. et al. (2010) Oncogene 29:5006. Entrez Accession # AAA81334. Bista, P. et al. (2010) J. Biol. Chem. 285:12971. van de Pavert, S.A. et al. (2010) Nat. Rev. Immunol. 10:664. Mouri, Y. et al. (2011) J. Immunol. 186:5047. Vondenhoff, M.F. et al. (2009) J. Immunol. 182:5439. Haybaeck, J. et al. (2009) Cancer Cell 16:295.
Long Name	Lymphotoxin beta Receptor
Entrez Gene IDs	4055 (Human); 17000 (Mouse); 297604 (Rat); 102135920 (Cynomolgus Monkey); 712550 (Rhesus Macaque)
Alternate Names	CD18, D12S370, LT-BETA-R, LTBR, Lymphotoxin beta R, Lymphotoxin-beta receptor, LymphotoxinbR, TNF RIII, TNF Rrp, TNF-R-III, TNFCRTNF-RIII, TNFR superfamily, member 3, TNFR-RP, TNFR2-RP, TNFR3, TNFRSF3, TNFRSF3TNFR-III, Tumor necrosis factor C receptor, Tumor necrosis factor receptor 2-related protein, Tumor necrosis factor receptor type III, ltbetar, lymphotoxin B receptor, lymphotoxin beta receptor (TNFR superfamily, member 3), tumor necrosis factor receptor superfamily member 3, tumor necrosis factor receptor superfamily, member 3

Citations for Recombinant Mouse Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

J Emgård et al. (2018), Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation Immunity, 48(1):120-132.e8.
PMID: 29343433
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Bando J et al. (2015), Identification and distribution of developing innate lymphoid cells in the fetal mouse intestine. Nat Immunol, 16(2):153-60.
PMID: 25501629

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