Skip to main content

Recombinant Mouse Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF

Bio-Techne includes R&D Systems | Catalog # 1008-LR

Catalog #
Availability
Size / Price
Qty
Loading...
1008-LR-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Lymphotoxin beta R/TNFRSF3 protein
Mouse Lymphotoxin betaR
(Ser28-Pro218)
Accession # P50284
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser28

Predicted Molecular Mass

48.7 kDa (monomer)

SDS-PAGE

63-67 kDa, reducing conditions

Activity

Measured by its ability to inhibit Lymphotoxin alpha1/ beta2-induced IL-8 secretion in A375 human melanoma cells. Degli-Esposti, M. et al. (1997) J. Immunol. 158:1756.
The ED50 for this effect is 15-75 ng/mL in the presence of 10 ng/mL of Recombinant Human Lymphotoxin alpha1/ beta2 (Catalog #
8884-LY).

Formulation, Preparation and Storage

1008-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin  betaR (LT betaR), previously called TNF RIII or TNF R‑related protein (TNF Rrp), is a type I transmembrane glycoprotein within the TNF receptor superfamily, designated TNFRSF3 (1, 2). Mouse LT betaR cDNA encodes 415 amino acids (aa) including a 30 aa signal peptide, a 193 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 171 aa cytoplasmic domain. The ECD contains four cysteine‑rich motifs characteristic of the TNF receptor superfamily (1). Within the ECD, mouse LT betaR shares aa sequence identity of 91% aa with rat, and 65-71% with human, canine, porcine, equine and bovine LT betaR. Soluble LT betaR can be formed by proteolytic cleavage of the ECD, and is an inhibitor of transmembrane LT betaR, as is recombinant LT betaR, which inhibits autoimmunity (2-5). A potential mouse isoform that ends at aa 218, just prior to the transmembrane domain, could also be secreted and inhibitory (6). LT betaR is expressed by visceral, lymphoid, and other stroma, epithelia and myeloid cells, but not lymphocytes (1, 3). LT betaR ligands include homotrimers of LIGHT (TNFSF14; also a ligand for HVEM) and the heterotrimeric lymphotoxin LT alpha1/ beta2 (2, 3, 5). Depending on the cell type and expression of TRAF3, activation of LT betaR has been shown to induce canonical (IKK/RelA; pro‑inflammatory) or alternative (NIK/RelB; lymphoid organogenic) NF kappaB activation (5, 7). LT betaR is expressed on mesenchymal stromal organizing cells that give rise to stroma of primary (thymus), secondary (tonsils, lymph nodes and Peyers patches) and tertiary (ectopic inflammatory) lymphoid structures (2-4, 8-10). Secondary immune tissues are absent in LT betaR‑deficient mice (2-4). LT betaR engagement induces production of IL‑7, RANK, TRANCE/RANK L, VEGF‑C, adhesion molecules such as VCAM‑1, ICAM‑1 and MAdCAM, and chemokines such as CXCL13, CCL19 and CCL21 (2, 8-10). LT betaR is expressed by hepatocytes, is up-regulated in regeneration, hepatitis and hepatocellular carcinoma, and influences lipid metabolism and atherosclerosis (3, 5, 11). It regulates cell growth and can initiate inflammation‑related carcinogenesis (5, 11).

References

  1. Force, W.R. et al. (1995) J. Immunol. 155:5280.
  2. McCarthy, D.D. (2006) Immunol. Res. 35:41.
  3. Tumanov, A.V. et al. (2007) Curr. Mol. Med. 7:567.
  4. Boehm, T. et al. (2003) J. Exp. Med. 198:757.
  5. Wolf, M.J. et al. (2010) Oncogene 29:5006.
  6. Entrez Accession # AAA81334.
  7. Bista, P. et al. (2010) J. Biol. Chem. 285:12971.
  8. van de Pavert, S.A. et al. (2010) Nat. Rev. Immunol. 10:664.
  9. Mouri, Y. et al. (2011) J. Immunol. 186:5047.
  10. Vondenhoff, M.F. et al. (2009) J. Immunol. 182:5439.
  11. Haybaeck, J. et al. (2009) Cancer Cell 16:295.

Long Name

Lymphotoxin beta Receptor

Alternate Names

LTBR, LymphotoxinbR, TNF RIII, TNF Rrp, TNFRSF3

Entrez Gene IDs

4055 (Human); 17000 (Mouse); 297604 (Rat); 102135920 (Cynomolgus Monkey); 712550 (Rhesus Macaque)

Gene Symbol

LTBR

UniProt

Additional Lymphotoxin beta R/TNFRSF3 Products

Product Documents for Recombinant Mouse Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF

For research use only

Loading...
Loading...
Loading...