Product Specifications for Recombinant Mouse LRRN4 His-tag Protein, CF
Mouse myeloma cell line, NS0-derived mouse LRRN4 protein Gln22-Ser676, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Gln22 inferred from enzymatic pyroglutamate treatment revealing Ser23
Predicted Molecular Mass
110-130 kDa, under reducing conditions
Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse LRRN4 His-tag, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.
Scientific Data Examples for Recombinant Mouse LRRN4 His-tag Protein, CF
Recombinant Mouse LRRN4 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Mouse LRRN4 His-tag (Catalog # 10411-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110-130 kDa./td/tr/tbody/table>
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 1 mg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
LRRN4 (Leucine-rich repeat neuronal protein 4) is a type I transmembrane protein that is a member of the LRRN family. It is expressed in lung, heart, ovary, and neuronal tissues (1-3). Mature mouse LRRN4 is composed of a 657 amino acid (aa) extracellular domain (ECD) that includes ten LRRs, one LRRCT and a fibronectin type-III like domain, a 21 aa transmembrane segment, and a 36 aa cytoplasmic domain. Within the ECD, mouse LRRN4 shares 66% and 84% aa sequence identity with human and rat LRRN4, respectively. LRRN4-deficient mice show defects in the memory retention, suggesting this protein may play an important role in hippocampus-dependent long-lasting memory (1). In addition, LRRN4 is found in about 8% dorsal root ganglion (DRG) neurons (2). These neurons are small-sized neurons that function as nociceptors. LRRN4 expression was decreased in the DRG by sciatic axotomy suggesting that LRRN4 might function as a synaptic adhesion molecule to maintain nociceptive circuits (2). LRRN4 is also expressed in primary mesothelial cells and may be developed as a maker for detection of mesothelioma antigens (4).
Bando, T. et al. (2005) Mol. Cell. Biol. 25:4166.
Bando, T. et al. (2012) Neurosci. Lett. 531:24.
Bando, T. et al. (2013) Neurosci. Lett. 548:73.
Kanamori-Katayama, M. (2011) PloS. One. 6(10):e24391.
Leucine-rich Repeat Neuronal 4
Entrez Gene IDs
164312 (Human); 320974 (Mouse); 311443 (Rat)
C20orf75, FLJ23994, LRRN4, MGC25027, NLRR-4, NLRR4, Neuronal leucine-rich repeat protein 4, chromosome 20 open reading frame 75, dJ1056H1.1, leucine rich repeat neuronal 4, leucine-rich repeat neuronal protein 4
Citations for Recombinant Mouse LRRN4 His-tag Protein, CF
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Product Documents for Recombinant Mouse LRRN4 His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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