Product Specifications for Recombinant Mouse IGSF4A/SynCAM1 Protein, CF
Mouse myeloma cell line, NS0-derived mouse IGSF4A/SynCAM1 protein Ile42-Asp376, with a C-terminal 10-His tag
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
76-87 kDa, reducing conditions
Measured by its binding activity in a functional ELISA. Galiberet, L. et al. (2005) J. Biol. Chem. 280:21955. Immobilized Recombinant Mouse CRTAM Fc Chimera (Catalog # 3687-CR) at 2 µg/mL (100 µL/well) can bind Recombinant Mouse IGSF4A/SynCAM1 with a linear range of 0.2-10 µg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
IGSF4A is a 75 kDa immunoglobulin superfamily protein that mediates calcium-independent adhesive interactions, either homophilic or heterophilic with CRTAM (class I-restricted T cell-associated molecule), Necl-1 (SynCAM3) or Nectin-3 (1 - 4). Alternate names for IGSF4A reflect its activities. As TSLC-1 (tumor suppressor in lung cancer-1), it is downregulated in non-small cell lung cancer (5). This may allow evasion of antitumor responses, since TSLC-1 interaction with CRTAM on activated NK and CD8+ T cells stimulates cytotoxicity and upregulates IFN-gamma production (3, 6). As Necl-2 (nectin-like 2), it is expressed on T zone-restricted BDCA3+ dendritic cells, again interacting with CRTAM (3). As SynCAM-1 (synaptic cell adhesion molecule-1) it binds homotypically across synaptic clefts and, like neuroligin/neurexin adhesion pairs, promotes formation of neural cell synapses (7, 8). Expression on mast cells allows attachment to neurites and fibroblasts (9, 10). As SgIGSF (spermatogenic IgSF), expression on developing sperm allows adhesion to an unknown partner on Sertoli cells that is critical for normal development (11, 12). Mouse IGSF4A cDNA encodes 445 amino acids including a signal peptide, three Ig-like domains (a V-type, a divergent C1-type and an I-type), a transmembrane domain and a cytoplasmic domain with protein 4.1 and PDZ domain binding sites. These sites allow connections to adaptor molecules and are critical in synaptogenesis (7, 8). The outer Ig-like domain most likely mediates adhesion, with altered affinity for splice variants that have either shorter spacing of this domain from the plasma membrane or varied glycosylation (1, 6). The six reported splice variants include a prominently expressed soluble form of about 45 kDa that inhibits adhesion (13). Mouse IGSF4A ECD shares 99%, 96%, 98% and 88% amino acid identity with rat, human, canine, and bovine IGSF4A, respectively.
Biederer, T. et al. (2006) Genomics 87:139.
Wakayama, T. et al. (2001) Mol. Reprod. Dev. 60:158.
Galibert, L. et al. (2005) J. Biol. Chem. 280:21955.
Shingai, T. et al. (2003) J. Biol. Chem. 278:35421.
Kuramochi, M. et al. (2002) Nat. Genet. 27:427.
Boles, K.S. et al. (2005) Blood 106:779.
Biederer, T. et al. (2002) Science 297:1525.
Sara, Y. et al. (2005) J. Neurosci. 25:260.
Furuno, T. et al. (2005) J. Immunol. 174:6934.
Ito, A. et al. (2003) Blood 101:2601.
Wakayama, T. et al. (2001) Mol. Reprod. Dev. 60:158.
Fujita, E. et al. (2006) Mol. Cell. Biol. 26:718.
Koma, Y. et al. (2004) Oncogene 23:5687.
Immunoglobulin Superfamily, Member 4
Entrez Gene IDs
23705 (Human); 54725 (Mouse)
BL2, Cadm1, IGSF4A, IGSF4ADKFZp686F1789, IGSF4immunoglobulin superfamily, member 4D variant 2, IgSF4, Immunoglobulin superfamily member 4, NECL2MGC149785, Necl-2, Nectin-like protein 2, RA175, ST17, SYNCAMMGC51880, Spermatogenic immunoglobulin superfamily, SynCAM, SynCAM1, Synaptic cell adhesion molecule, TSLC-1, TSLC1, TSLC1/Nectin-like 2/IGSF4, Tumor suppressor in lung cancer 1SYNCAM1, cell adhesion molecule 1, immunoglobulin superfamily, member 4, nectin-like 2, sTSLC-1, sgIGSF
Citations for Recombinant Mouse IGSF4A/SynCAM1 Protein, CF
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Product Documents for Recombinant Mouse IGSF4A/SynCAM1 Protein, CF
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