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Recombinant Mouse ICAM-5 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1173-M5

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1173-M5-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse ICAM-5 protein
Mouse ICAM-5
Leu31-Arg828 (Pro47Arg)
Accession # Q60625.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu31

Predicted Molecular Mass

112 kDa (monomer)

SDS-PAGE

166 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
When 5 x 104 cells/well are added to recombinant mouse ICAM-5 Fc Chimera coated plates (25 µg/mL with 100 µL/well), >25% cells will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

1173-M5
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution
Reconstitute at 400 μg/mL in sterile PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-5

Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is an integral membrane glycoprotein expressed in neurons of mammalian telencephalons. ICAM-5 is a member of the immunoglobulin superfamily and shares 38 - 55% amino acid homology with other ICAMs. Structurally, ICAM-5 contains nine Ig domains that included 15 N‑glycosylation sites, a single transmembrane region, and C-terminal cytoplasmic tail (1).

As with other members of the ICAM family, ICAM-5 has been shown to be involved in cellular adhesion. ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) via its first NH2-terminal Ig domain. The ability of ICAM-5 to bind LFA-1 suggests that ICAM-5 may play an important role in immune responses in the central nervous system (2). Additionally, ICAM-5 has been found to promote homophilic binding via binding of the first Ig domain to Ig domains 4 - 5. Homophilic adhesion activity of ICAM-5 is regulated by a monomer/tetramer transition. ICAM-5 expression temporally parallels the onset of dendritic elongation and synaptogenesis during the postnatal period suggesting that ICAM-5 may provide a brain segment-specific cue for synaptogenesis or dendrite-dendrite interaction (3).

References

  1. Tian, L. et al. (2000) Eur. J. Immunol. 30:810.
  2. Mizuno, T. and K. Mori (1997) J. Biol. Chem. 272:1156.
  3. Tian, L. et al. (2000) J. Cell Biol. 150:243.

Long Name

Intercellular Adhesion Molecule 5

Alternate Names

ICAM5, Telencephalin

Entrez Gene IDs

7087 (Human); 15898 (Mouse)

Gene Symbol

ICAM5

UniProt

Additional ICAM-5 Products

Product Documents for Recombinant Mouse ICAM-5 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse ICAM-5 Fc Chimera Protein, CF

For research use only

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Reconstitution Calculator

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