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Recombinant Mouse gAdiponectin/gAcrp30 Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1119-AC

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1119-AC-025

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse gAdiponectin/gAcrp30 protein
Ala111-Asn247 & Tyr112-Asn247, both with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala111 & Tyr112

Predicted Molecular Mass

16.7 kDa (monomer)

SDS-PAGE

18-24 kDa, reducing conditions

Activity

Measured by its ability to induce TIMP-1 secretion by mouse macrophages. Kumada, M. et al. (2004) Circulation 109:2046.
The ED50 for this effect is 5-20 µg/mL.

Formulation, Preparation and Storage

1119-AC
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: gAdiponectin/gAcrp30

Adiponectin, alternatively named Adipocyte Complement-Related Protein of 30 kDa (Acrp30), shares structural similarity with complement factor C1q and is a member of the family of defense collagens (1 - 7). It is secreted exclusively by differentiated adipocytes and circulates at high concentrations (in μg/mL range). Adiponectin has a modular structure comprising an N-terminal collagenous domain with multiple collagen triple helix repeats, followed by a C-terminal C1q-like globular domain. The globular domain has similar folding topology with tumor necrosis factor-alpha and assembles into homotrimers. Higher order oligomeric adiponectins (hexamers and higher molecular weight forms) are also formed via interactions between the collagenous stalk. A truncated form of Adiponectin containing only the globular domain (gAdiponectin or gAcrp30) can be generated by proteolytic cleavage (5). The gAdiponectin as well as all oligomeric forms of the full length Adiponectin are detected in serum. Different isoforms of Adiponectin have been shown to activate different signal transduction pathways (8 - 11). Conflicting biological activities have been reported for the various isoforms.

Two seven membrane-spanning Adiponectin receptors, designated AdipoR1 and AdipoR2 have been identified (12). AdipoR1 is expressed predominantly in muscle and functions as a high‑affinity receptor for gAdiponectin, but a very low-affinity receptor for the full length Adiponectin. AdipoR2 binds both the full length and globulin domain with intermediate affinity and is expressed primarily in liver.

Adiponectin is an anti-diabetic and anti-atherogenic hormone that plays important roles in the regulation of lipid and glucose metabolism (1 - 7). Similarly to full length Adiponectin, recombinant gAdiponectin from R&D Systems has been shown to inhibit proliferation of mouse M1 myeloid cells (13). The 137 amino acid (aa) residue globular domain of mouse Adiponectin shares 89% aa sequence identity with the human homologue.

References

  1. Scherer, P.E. et al. (1995) J. Biol. Chem. 270:26746.
  2. Hu, E. et al. (1996) J. Biol. Chem. 271:10697.
  3. Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286.
  4. Tsao, T-S. et al. (2002) J. Biol. Chem. 277:29359.
  5. Fruebis, J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2005.
  6. Berg, A.H. et al. (2001) Nature Med. 7:947.
  7. Pajvani, U.T. et al. (2003) J. Biol. Chem. 278:9073.
  8. Tomas, E. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16309.
  9. Tsao, T.S. et al. (2003) J. Biol. Chem. Sept. 30 [Epub ahead of print].
  10. Wang, Y. et al. (2002) J. Biol. Chem. 277:19521.
  11. Sato, C. et al. (2001) J. Biol. Chem. 276:28849.
  12. Yamauchi, T. et al. (2003) Nature 423:762.
  13. Yokota, T. et al. (2000) Blood 96:1723.

Alternate Names

gAcrp30, gAdipoQ, gApM1

Entrez Gene IDs

9370 (Human); 11450 (Mouse); 246253 (Rat)

Gene Symbol

ADIPOQ

UniProt

Additional gAdiponectin/gAcrp30 Products

Product Documents for Recombinant Mouse gAdiponectin/gAcrp30 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse gAdiponectin/gAcrp30 Protein, CF

For research use only

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