Mouse myeloma cell line, NS0-derived mouse FLRT1 protein Thr52-Pro552
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
65-83 kDa, under reducing conditions.
Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. The ED50 for this effect is 0.50-4.00 μg/mL.
Recombinant Mouse FLRT1 His-tag Protein, CF Scientific Data Examples
Recombinant Mouse FLRT1 His-tag Protein Bioactivity.
Recombinant Mouse FLRT1 His-tag Protein (Catalog # 10955-FL) supports the adhesion of Neuro 2A mouse neuroblastoma cells. The ED50 for this effect is 0.500-4.00 µg/mL.
Recombinant Mouse FLRT1 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Mouse FLRT1 His-tag Protein (Catalog # 10955-FL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-83 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Fibronectin-like domain-containing leucine-rich transmembrane protein 1 (FLRT1) is one of three FLRT leucine-rich repeat transmembrane glycoproteins involved in the regulation of FGF signaling. FLRTs are characterized by an extracellular domain (ECD) with fibronectin and leucine rich repeat (LRR) motifs involved in cell adhesion functions (1). Mature mouse FLRT1 consists of an ECD with 10 N-terminal leucine-rich repeats and a juxtamembrane fibronectin type III domain, a single transmembrane domain and a short cytoplasmic domain lacking defined signaling motifs (1). The ECD of mouse FLRT1 shares 97% amino acid (aa) sequence identity with the ECD of human FLRT1. Both during development and in adulthood, FLRT1 is expressed in distinct areas of the brain and other tissues, including the kidney, and Its expression is distinct from that of FLRT2 and FLRT3 (1, 2). In mouse, FLRT1 is expressed at brain compartmental boundaries in embryos (2). The fibronectin domain of all three FLRTs can bind FGF receptors and this binding is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization of FLRTs in areas of cell contact and homotypic cell-cell association (4). This may be through direct interactions with other FLRT molecules or, as has been shown for FLRT3, by regulating internalization of adhesion molecules such as cadherins (4, 5). Down-regulation of FLRT1 has been associated with prognosis of gastric cancer and has potential to be a therapeutic target for tumor treatment (6).
Lacy, S.E. et al. (1999) Genomics 62:417.
Haines, B.P. et al. (2006) Dev. Biol. 297:14.
Bottcher, R.T. et al. (2004) Nat. Cell Biol. 6:38.
Karaulanov, E.E. et al. (2006) EMBO Rep. 7:283.
Ogata, S. et al. (2007) Genes Dev. 21:1817.
Liang, Y. et al. (2019) Pathol. Res. Pract. 215:152570.
Fibronectin Leucine Rich Transmembrane Protein 1
Entrez Gene IDs
23769 (Human); 396184 (Mouse)
FLRT1, Fibronectin-like domain-containing leucine-rich transmembrane protein 1, MGC21624, SPG68, fibronectin leucine rich transmembrane protein 1, leucine-rich repeat transmembrane protein FLRT1
Citations for Recombinant Mouse FLRT1 His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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