Recombinant Mouse FGF-10 Protein

Name: Recombinant Mouse FGF-10 Protein
Brand: R&D Systems
SKU: 6224-FG

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 6224-FG

Citations (27)

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Key Product Details

Source:	E. coli
Accession #:	NP_032028.1
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse FGF-10 Protein

Source	E. coli-derived mouse FGF-10 protein Ser62-Thr209
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser62
Predicted Molecular Mass	17.1 kDa
SDS-PAGE	17 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED₅₀ for this effect is 20-120 ng/mL

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

6224-FG

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄, EDTA and DTT with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6224-FG/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄, EDTA and DTT.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: FGF-10

The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22 (1). Mature mouse FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). C-terminal to this region (aa 62 ‑ 209), it shares 94% and 100% amino acid sequence identity with human and rat FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP (1, 4). It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) (5). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation (6), palate development (7), branching morphogenesis and directional outgrowth of lung buds (2, 8), formation of the otic vesicle and chochlea (9), adipogenesis (10), and the development of prostate, mammary, lacrimal, and submandibular salivary glands (11 - 14). FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis (7, 9, 12 ‑ 14). The expression and function of FGF‑10 are negatively regulated by Shh and BMP-4 in the developing lung (2, 8). Overlapping expression patterns and activities with FGF-3, -7, and -8 suggest at least a partial redundancy in FGF-10 biology (7, 9, 13, 14). FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer (15).

Item	Value
References	Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235. Bellusci, S. et al. (1997) Development 124:4867. Tagashira, S. et al. (1997) Gene 197:399. Beer, H.-D. et al. (2005) Oncogene 24:5269. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694. Min, H. et al. (1998) Genes Dev. 12:3156. Rice, R. et al. (2004) J. Clin. Invest. 113:1692. Weaver, M. et al. (2000) Development 127:2695. Pirvola, U. et al. (2000) J. Neurosci. 20:6125. Sakaue, H. et al. (2002) Genes Dev. 16:908. Donjacour, A.A. et al. (2003) Dev. Biol. 261:39. Mailleux, A.A. et al. (2002) Development 129:53. Makarenkova, H.P. et al. (2000) Development 127:2563. Jaskoll, T. et al. (2005) BMC Dev. Biol. 5:11. Nomura, S. et al. (2008) Br. J. Cancer 99:305.
Long Name	Fibroblast Growth Factor 10
Entrez Gene IDs	2255 (Human); 14165 (Mouse); 25443 (Rat)
Alternate Names	FGF-10, FGF10, KGF-2, KGF2, Keratinocyte growth factor 2, fibroblast growth factor 10, produced by fibroblasts of urinary bladder lamina propria

Citations for Recombinant Mouse FGF-10 Protein (27)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse FGF-10 Protein include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

H Kim et al. (2022), Ultraefficient extracellular vesicle-guided direct reprogramming of fibroblasts into functional cardiomyocytes Science Advances, 8(8):eabj6621.
PMID: 35213232
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

N Sankoda et al. (2021), Epithelial expression of Gata4 and Sox2 regulates specification of the squamous-columnar junction via MAPK/ERK signaling in mice Nature Communications, 12(1):560.
PMID: 33495473
Species: Mouse
Sample Types: Reference Standard

H Tomita et al. (2021), Inhibition of FGF10-ERK signal activation suppresses intraductal papillary neoplasm of the bile duct and its associated carcinomas Cell Reports, 34(8):108772.
PMID: 33626352
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

N Hosaka et al. (2021), Induction of gammadeltaT cells from HSC-enriched BMCs co-cultured with iPSC-derived thymic epithelial cells Journal of Cellular and Molecular Medicine, 0(0).
PMID: 34687276
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

KA Lloyd et al. (2020), Netazepide Inhibits Expression of Pappalysin 2 in Type-1 Gastric Neuroendocrine Tumors Cell Mol Gastroenterol Hepatol, 0(0).
PMID: 32004755
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

H Katsura et al. (2020), Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction Cell Stem Cell, 0(0).
PMID: 33128895
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Kiyokawa et al. (2020), Drug-Induced Naï¿½ve iPS Cells Exhibit Better Performance than Primed iPS Cells with Respect to the Ability to Differentiate into Pancreatic &beta-Cell Lineage Journal of Clinical Medicine, 9(9).
PMID: 32887316
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

T Yung et al. (2019), Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals Nat Commun, 10(1):4647.
PMID: 31604927
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

T Momozane et al. (2019), Efficient Differentiation of Mouse Induced Pluripotent Stem Cells into Alveolar Epithelium Type II with a BRD4 Inhibitor Stem Cells Int, 2019(0):1271682.
PMID: 31949433
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

I Guerriero et al. (2019), Exploring the Molecular Crosstalk between Pancreatic Bud and Mesenchyme in Embryogenesis: Novel Signals Involved Int J Mol Sci, 20(19).
PMID: 31623299
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Westphal et al. (2019), Fibroblast growth factor 8b induces uncoupling protein 1 expression in epididymal white preadipocytes Sci Rep, 9(1):8470.
PMID: 31186471
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

C Miyagi-Shi et al. (2018), Characterization of induced tissue-specific stem cells from pancreas by a synthetic self-replicative RNA Sci Rep, 8(1):12341.
PMID: 30120295
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

C Zhong et al. (2018), Barhl1 is required for the differentiation of inner ear hair cell-like cells from mouse embryonic stem cells Int. J. Biochem. Cell Biol., 96(0):79-89.
PMID: 29413750
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

L Chatzeli et al. (2017), Fgf10-Sox9 are essential for establishment of distal progenitor cells during salivary gland development Development, 0(0).
PMID: 28506998
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

KW McCracken et al. (2017), Wnt/?-catenin promotes gastric fundus specification in mice and humans Nature, 0(0).
PMID: 28052057
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

N Cerdá-Este et al. (2017), Stepwise reprogramming of liver cells to a pancreas progenitor state by the transcriptional regulator Tgif2 Nat Commun, 8(0):14127.
PMID: 28193997
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

MA Scavuzzo et al. (2017), Organotypic pancreatoids with native mesenchyme develop Insulin producing endocrine cells Sci Rep, 7(1):10810.
PMID: 28883507
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Jennifer Quantius (2016), Influenza Virus Infects Epithelial Stem/Progenitor Cells of the Distal Lung: Impact on Fgfr2b-Driven Epithelial Repair PLoS Pathog, 12(6):e1005544.
PMID: 27322618
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2016), Tissue-Specific Stem Cells Obtained by Reprogramming of Non-Obese Diabetic (NOD) Mouse-Derived Pancreatic Cells Confer Insulin Production in Response to Glucose PLoS ONE, 11(9):e0163580.
PMID: 27662374
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Marie-Isabelle Garcia (2016), Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage Development, 143(9):1452-63.
PMID: 26989172
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Yang Z et al. (2015), Mesenchymal Wnt/beta-Catenin Signaling Controls Epithelial Stem Cell Homeostasis in Teeth by Inhibiting the Antiapoptotic Effect of Fgf10. Stem Cells, 33(5):1670-81.
PMID: 25693510
Noguchi et al. (2015), Induction of tissue-specific stem cells by reprogramming factors, and tissue-specific selection. Cell Death Differ, 22(1):145-55.
PMID: 25190146
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Chan W et al. (2015), 2-O Heparan Sulfate Sulfation by Hs2st Is Required for Erk/Mapk Signalling Activation at the Mid-Gestational Mouse Telencephalic Midline. PLoS ONE, 10(6):e0130147.
PMID: 26075383
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Cai Z et al. (2014), Role of heparan sulfate proteoglycans in optic disc and stalk morphogenesis. Dev Dyn, 243(10):1310-6.
PMID: 24753163
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Song Z et al. (2013), Mice with Tak1 deficiency in neural crest lineage exhibit cleft palate associated with abnormal tongue development. J Biol Chem, 288(15):10440-50.
PMID: 23460641
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Sneddon et al. (2012), Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme. Nature, 491(7426):765-8.
PMID: 23041930
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Rebustini IT et al. (2012), miR-200c regulates FGFR-dependent epithelial proliferation via Vldlr during submandibular gland branching morphogenesis. Development, 139(1):191-202.
PMID: 22115756

Recombinant Mouse FGF-10 Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Mouse FGF-10 Protein

Formulation, Preparation and Storage

Carrier Free

6224-FG

Carrier

6224-FG/CF

Carrier Free

Reconstitution Calculator

Background: FGF-10

Citations for Recombinant Mouse FGF-10 Protein (27)

Product Documents for Recombinant Mouse FGF-10 Protein

Product Datasheets

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