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Recombinant Mouse EDA-A1/Ectodysplasin A1 Protein

Bio-Techne includes R&D Systems | Catalog # 191-ED

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Carrier Free
191-ED-010/CF

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With Carrier
191-ED-010

Key Product Details

Source

NS0

Accession #

Structure / Form

Homotrimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse EDA-A1/Ectodysplasin A1 protein
Ala179-Ser391, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

23 kDa

SDS-PAGE

27-37 kDa, reducing conditions

Activity

Measured by its ability to compete with biotinylated rhEDA-A1 for binding to immobilized rhEDAR/Fc Chimera.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 191-ED
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 191-ED/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDA-A1/Ectodysplasin A1

Ectodysplasin is a 45 kDa type II transmembrane TNF superfamily protein that is associated with X-linked hypohidrotic ectodermal dysplasia (HED), a disorder of hair, tooth, and sweat gland development (1 - 4). The mouse EDA-A1 cDNA encodes a 41 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 329 extracellular region that contains a terminal TNF homology domain, a collagenous domain, and a stalk region (5 - 8). Within the collagenous and TNF homology domains, mouse EDA-A1 shares greater than 97% aa  sequence identity with bovine, canine, human, and rat EDA-A1. Multiple alternately spliced EDA variants have been described (4, 9). The dominant variant, EDA-A2, has a deletion of two amino acids that changes the receptor binding selectivity from EDAR to XEDAR (4, 9, 10). The collagenous domain of EDA-A1 mediates noncovalent homotrimer formation (7, 8). Shedding of the collagenous and TNF homology domains of EDA-A1 is accomplished by a furin-like protease. The released fragment maintains its trimeric state and ability to bind EDAR (11, 12). Some EDA-A1 polymorphisms found in HED patients alter the protease recognition site and prevent shedding (11). EDA-A1 is expressed in developing hair follicles, epidermis, teeth, sweat glands, salivary glands, and forebrain (8, 10, 13 - 15). It regulates ectodermal appendage formation and is critical to the patterning and morphogenesis of hair follicles, partially through the induction of Lymphotoxin beta (7, 14, 16). Receptor and ligand expression are regulated by factors involved in many aspects of tissue morphgenesis. EDA-A1 expression is induced by Wnt6, (14, 15) while the expression of EDAR is induced by Activin  betaA and inhibited by BMP-2, -4, and -7 (15, 17).

References

  1. Mikkola, M.L. and I. Thesleff (2003) Cytokine Growth Factor Rev. 14:211.
  2. Botchkarev, V.A. and M.Y. Fessing (2005) J. Invest. Dermatol. 10:247.
  3. Kere, J. et al. (1996) Nat. Genet. 13:409.
  4. Bayes, M. et al. (1998) Hum. Mol. Genet. 7:1661.
  5. Ferguson, B.M. et al. (1997) Hum. Mol. Genet. 6:1589.
  6. Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. 94:13069.
  7. Ezer, S. et al. (1999) Hum. Mol. Genet. 8:2079.
  8. Mikkola, M.L. et al. (1999) Mech. Dev. 88:133.
  9. Hashimoto, T. et al. (2006) Gene 371:42.
  10. Yan, M. et al. (2000) Science 290:523.
  11. Chen, Y. et al. (2001) Proc. Natl. Acad. Sci. 98:7218.
  12. Elomaa, O. et al. (2001) Hum. Mol. Genet. 10:953.
  13. Pispa, J. et al. (2003) Gene Exp. Patterns 3:675.
  14. Laurikkala, J. et al. (2002) Development 129:2541.
  15. Laurikkala, J. et al. (2001) Dev. Biol. 229:443.
  16. Cui, C.Y. et al. (2006) Proc. Natl. Acad. Sci. 103:9142.
  17. Mou, C. et al. (2006) Proc. Natl. Acad. Sci. 103:9075.

Alternate Names

EDAA1

Entrez Gene IDs

1896 (Human); 13607 (Mouse)

Gene Symbol

EDA

UniProt

Additional EDA-A1/Ectodysplasin A1 Products

Product Documents for Recombinant Mouse EDA-A1/Ectodysplasin A1 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse EDA-A1/Ectodysplasin A1 Protein

For research use only

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