Key Product Details
(Ala26 - His637)
Accession # NP_690879
(Pro100 - Lys330)
N-terminal Sequence Analysis
Predicted Molecular Mass
When 4 x 104 neurons/well are added to 96-well plate containing serial dilutions of Recombinant Mouse DNER Fc Chimera and immobilized Recombinant Mouse Pleiotrophin/PTN (1 μg/mL) (Catalog # 6580-PL), neurite outgrowth is significantly inhibited in a dose dependent manner. The ED50 for this effect is 0.5 ‑ 2.5 μg/mL.
Formulation, Preparation and Storage
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
Reconstitute at 200 μg/mL in PBS.
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
DNER (Delta/Notch-like EGF-related receptor), also known as BET (brain-specific EGF-like transmembrane protein), is a type I transmembrane glycoprotein of the Notch/Delta family (1, 2). Mouse DNER has been detected as 90, 120 and 150 kDa forms which are probably variably glycosylated (1, 2). DNER is specifically expressed on nonaxonal areas of post-mitotic neurons, especially Purkinje cells, but also cortical and hippocampal pyramidal neurons and immature cerebellar granule cells (1 ‑ 5). After expression on the cell surface, DNER is removed from axonal membranes, creating its somatodendritic specificity (1, 3, 6). A portion of DNER is found within endosomes (1, 3, 6). Mouse DNER cDNA encodes 737 amino acids (aa) that include a 25 aa signal sequence, a 615 aa extracellular domain (ECD) containing ten distinct Delta/Notch-like EGF-like repeats, a 21 aa transmembrane sequence, and a 76 aa cytoplasmic domain. The mouse DNER ECD shares 89%, 96% and 86% aa sequence identity with human, rat and bovine DNER, respectively. DNER is a Notch ligand, but is considered a non-classical ligand because it lacks the usual DSL Notch binding motif (5, 7). Instead, Notch interacts with the two EGF-like repeats closest to the N-terminus of DNER (5). DNER also associates with protein tyrosine phosphatase zeta (PTP zeta), which is the receptor of pleiotrophin (PTN). PTP zeta-PTN-DNER signaling has been implicated in the regulation of neuritogenesis (3). Mice lacking DNER show impaired cerebellar functions and delayed Purkinje cell-mediated maturation of Notch-expressing Bergmann glia during cerebellar development (4, 5). Expression of DNER in glioblastoma stem-like cells inhibits formation of neurospheres in vitro, induces differentiation and inhibits growth of xenografts, thus acting as a tumor suppressor (7). Expression of DNER in adipose‑derived human mesenchymal stem cells and mouse auditory hair cells have also been shown (8, 9).
- Eiraku, M. et al. (2002) J. Biol. Chem. 277:25400.
- Nishizumi, H. et al. (2002) NeuroReport 13:909.
- Fukazawa, N. et al. (2008) Mol. Cell. Biol. 28:4494.
- Tohgo, A. et al. (2005) Mol. Cell. Neurosci. 31:326.
- Eiraku, M. et al. (2005) Nat. Neurosci. 8:873.
- Kurisu, J. et al. (2010) J. Neurochem. 113:1598.
- Sun, P. et al. (2009) Stem Cells 27:1473.
- Park, J-R. et al. (2010) Cell Prolif. 43:19.
- Hartman, B.H. et al. (2010) J. Assoc. Res. Otolaryngol. 11:187.
Product Specific Notices for Recombinant Mouse DNER Fc Chimera Protein, CF
For research use only