Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Recombinant Mouse Cystatin C Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1238-PI

Catalog #
Availability
Size / Price
Qty
Loading...
1238-PI-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Inhibition Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Cystatin C protein
Met1-Ala140, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21

Predicted Molecular Mass

15 kDa

SDS-PAGE

15 kDa, 18 kDa, 20 kDa and 21 kDa, reducing conditions

Activity

Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (Catalog # ES009).
The IC50 value is <10 nM, under the described conditions.

Formulation, Preparation and Storage

1238-PI
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile 25 mM HEPES and 150 mM NaCl, pH 7.8.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin C

Cystatin C is a member of family 2 of the cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). All mouse tissues analyzed expressed Cystatin C, with relative levels similar to those of rat and human tissues. For all three species, brain and liver had the highest and lowest levels of Cystatin C, respectively, whereas kidney, spleen and muscle had the levels in between (4). The high degree of similarity in distribution and functional properties for mouse, rat and human Cystatin C indicates that a murine model should be relevant for studies of the human disease, hereditary Cystatin C amyloid angiopathy (4).

References

  1. Reed, C.H. (2000) British J. Biomed. Sci. 57:323.
  2. Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.
  3. Abrahamson, M. (1994) Methods Enzymol. 244:685.
  4. Hakansson, K. et al. (1996) Comp. Biochem. Physiol. 114B:303.

Alternate Names

ARMD11, CST3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin

Entrez Gene IDs

1471 (Human); 13010 (Mouse); 25307 (Rat)

Gene Symbol

CST3

UniProt

Additional Cystatin C Products

Product Documents for Recombinant Mouse Cystatin C Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Cystatin C Protein, CF

For research use only

Citations for Recombinant Mouse Cystatin C Protein, CF (2)

Showing  1 - 2 of 2 publications Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Cystatin C Protein, CF include:

There are no citations that match your criteria.

Showing  1 - 2 of 2 publications Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=