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Recombinant Mouse Cystatin C Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1238-PI

Catalog #
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Key Product Details



Accession #




Inhibition Activity

Product Specifications


Mouse myeloma cell line, NS0-derived mouse Cystatin C protein
Met1-Ala140, with a C-terminal 10-His tag


>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis


Predicted Molecular Mass

15 kDa


15 kDa, 18 kDa, 20 kDa and 21 kDa, reducing conditions


Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (Catalog # ES009).
The IC50 value is <10 nM, under the described conditions.

Formulation, Preparation and Storage

Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile 25 mM HEPES and 150 mM NaCl, pH 7.8.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin C

Cystatin C is a member of family 2 of the cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). All mouse tissues analyzed expressed Cystatin C, with relative levels similar to those of rat and human tissues. For all three species, brain and liver had the highest and lowest levels of Cystatin C, respectively, whereas kidney, spleen and muscle had the levels in between (4). The high degree of similarity in distribution and functional properties for mouse, rat and human Cystatin C indicates that a murine model should be relevant for studies of the human disease, hereditary Cystatin C amyloid angiopathy (4).


  1. Reed, C.H. (2000) British J. Biomed. Sci. 57:323.
  2. Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.
  3. Abrahamson, M. (1994) Methods Enzymol. 244:685.
  4. Hakansson, K. et al. (1996) Comp. Biochem. Physiol. 114B:303.

Alternate Names

ARMD11, CST3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin

Entrez Gene IDs

1471 (Human); 13010 (Mouse); 25307 (Rat)

Gene Symbol



Additional Cystatin C Products

Product Documents for Recombinant Mouse Cystatin C Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Cystatin C Protein, CF

For research use only

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