Recombinant Mouse CLF-1/CLC Complex Protein, CF

Name: Recombinant Mouse CLF-1/CLC Complex Protein, CF
Brand: R&D Systems
SKU: 10088-CL

Catalog # 10088-CL

Product Datasheet / COA / SDS

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Key Product Details

Source:	CHO
Accession #:	Q9JM58 (CLF-1), Q9QZM3 (CLC)
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse CLF-1/CLC Complex Protein, CF

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse CLF-1/CLC Complex protein

Mouse CLF-1 (Gly40-Gly425) Accession # Q9JM58	SGGGGSGGGGSGGGGS	Mouse CLC (Leu28-Phe225) Accession # Q9QZM3	HHHHHH
N-terminus			C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gly40 & Ala41

Predicted Molecular Mass

67 kDa

SDS-PAGE

77-87 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells transfected with human CNTF R alpha. The ED₅₀ for this effect is 15-90 ng/mL.

Recombinant Mouse CLF-1/CLC Complex Protein, CF Scientific Data Examples

	Recombinant Mouse CLF-1/CLC Complex Protein SDS-PAGE 2 μg/lane of Recombinant Mouse CLF‑1/CLC Complex (Catalog # 10088-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 77-87 kDa and 160-180 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10088-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLF-1/CLC Complex

Cardiotrophin-like cytokine (CLC), also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3), is a member of the IL-6 family of cytokines (1, 2). CLC associates with the secreted soluble cytokine-like factor 1 (CLF-1), a member of the cytokine type I receptor family, to form the heteromeric composite cytokine CLF-1/CLC (3, 5). CLC can also form an alternate composite cytokine with soluble ciliary neurotrophic factor receptor alpha (CNTF R alpha ) (4). Co-expression of CLC with either CLF-1 or CNTF R alpha is required for the formation of the composite cytokines and for CLC secretion (3-5). CLF-1/CLC binds to the membrane-associated CNTF R alpha to initiate the heterodimerization between gp130 and leukemia inhibitory factor receptor (LIFR) and stimulate the PI 3-kinase and the MAP kinase activity. The CLF-1/CLC complex displays activities only on those cells expressing the functional tripartite receptor complex (5). Mouse CLF-1 is a 425 amino acid (aa) protein that contains a 33 aa signal sequence and a 392 aa chain. Mouse CLC is a 225 amino acid (aa) protein that contains a 27 aa signal sequence and a 198 aa chain. Human and mouse CLF-1 share 94% amino acid sequence identity. Human and mouse CLC shares 96% amino acid sequence identity. CLF-1/CLC supports the survival of embryonic motor and sympathetic neurons and has been shown to induce astrocytes differentiation of fetal neuroepithelial cells (4-6). Both CLF-1 and CLC are expressed in the embryo, suggesting that the composite cytokine may have an important role in nervous system development (5, 7). CLC has also been shown to regulate immune functions by stimulating B cell proliferation and Ig production (8).

Item	Value
References	Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458. Shi, Y. et al. (1999) Biochem. Biophys. Res. Commun. 262:132. Elson, G.C.A. et al. (1998) J. Immunol. 161: 1371. Plun-Favreau, H. et al. (2001) EMBO J. 20:1692. Elson, G.C.A. et al. (2000) Nature Neurosci. 3:867. Uemura, A. et al. (2002) Cytokine 18:1. Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476. Senaldi, G. et al. (2002) J. Immunol. 168:5690.
Entrez Gene IDs	9244 (Human)
Alternate Names	CLF-1/CLC Complex

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