Chinese Hamster Ovary cell line, CHO-derived mouse CLF-1/CLC Complex protein
Mouse CLF-1 (Gly40-Gly425) Accession # Q9JM58
Mouse CLC (Leu28-Phe225) Accession # Q9QZM3
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Gly40 & Ala41
Predicted Molecular Mass
77-87 kDa, reducing conditions
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells transfected with human CNTF R alpha. The ED50 for this effect is 15-90 ng/mL.
Recombinant Mouse CLF-1/CLC Complex Protein, CF Scientific Data Examples
Recombinant Mouse CLF-1/CLC Complex Protein SDS-PAGE
2 μg/lane of Recombinant Mouse CLF‑1/CLC Complex (Catalog # 10088-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 77-87 kDa and 160-180 kDa, respectively.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 250 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CLF-1/CLC Complex
Cardiotrophin-like cytokine (CLC), also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3), is a member of the IL-6 family of cytokines (1, 2). CLC associates with the secreted soluble cytokine-like factor 1 (CLF-1), a member of the cytokine type I receptor family, to form the heteromeric composite cytokine CLF-1/CLC (3, 5). CLC can also form an alternate composite cytokine with soluble ciliary neurotrophic factor receptor alpha (CNTF R alpha ) (4). Co-expression of CLC with either CLF-1 or CNTF R alpha is required for the formation of the composite cytokines and for CLC secretion (3-5). CLF-1/CLC binds to the membrane-associated CNTF R alpha to initiate the heterodimerization between gp130 and leukemia inhibitory factor receptor (LIFR) and stimulate the PI 3-kinase and the MAP kinase activity. The CLF-1/CLC complex displays activities only on those cells expressing the functional tripartite receptor complex (5). Mouse CLF-1 is a 425 amino acid (aa) protein that contains a 33 aa signal sequence and a 392 aa chain. Mouse CLC is a 225 amino acid (aa) protein that contains a 27 aa signal sequence and a 198 aa chain. Human and mouse CLF-1 share 94% amino acid sequence identity. Human and mouse CLC shares 96% amino acid sequence identity. CLF-1/CLC supports the survival of embryonic motor and sympathetic neurons and has been shown to induce astrocytes differentiation of fetal neuroepithelial cells (4-6). Both CLF-1 and CLC are expressed in the embryo, suggesting that the composite cytokine may have an important role in nervous system development (5, 7). CLC has also been shown to regulate immune functions by stimulating B cell proliferation and Ig production (8).
Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458.
Shi, Y. et al. (1999) Biochem. Biophys. Res. Commun. 262:132.
Elson, G.C.A. et al. (1998) J. Immunol. 161: 1371.
Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.
Elson, G.C.A. et al. (2000) Nature Neurosci. 3:867.
Uemura, A. et al. (2002) Cytokine 18:1.
Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
Senaldi, G. et al. (2002) J. Immunol. 168:5690.
Entrez Gene IDs
Currently there are no citations for Recombinant Mouse CLF-1/CLC Complex Protein, CF.
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