Source	Mouse myeloma cell line, NS0-derived mouse CD200 R1 protein Thr26 - Pro238, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Thr26
Predicted Molecular Mass	24 kDa
SDS-PAGE	50-70 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse CD200 R1 binds with an ED₅₀ of 12‑72 ng/mL.

Recombinant Mouse CD200R1 His-tag Protein, CF Scientific Data Examples

	Recombinant Mouse CD200R1 His-tag Protein Binding Activity When Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse CD200 R1 (Catalog # 10054-CD) binds with an ED₅₀ of 12-72 ng/mL.
	Recombinant Mouse CD200R1 His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Mouse CD200 R1 His-tag (Catalog # 10054-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50 - 70 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10054-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD200R1

CD200 R1, also known as OX-2 receptor, is a 90 kDa, type I transmembrane protein that belongs to the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity (1-3). The mouse CD200 R1 cDNA encodes a 326 aa precursor that includes a signal sequence, a 213 aa extracellular domain (ECD), a single transmembrane segment, and a cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, mouse CD200 R1 shares 56% and 70% aa sequence identity with human and rat CD200 R1, respectively. The ECD of mouse CD200 R1 shares 69%, 38%, 79%, and 83% aa sequence identity with the ECD of mouse CD200 R2, 3, 4, and a CD200 R-like molecule, respectively. CD200 R1 is expressed primarily on mast cells, basophils, macrophages, and dendritic cells, (5-7) while its ligand, CD200, is widely distributed (8). Disruption of this receptor-ligand pair by knockout of the CD200 gene leads to increased macrophage number and activation, plus a predisposition to autoimmune disorders (9). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (10). The CD200 R-like molecules may interact differently with CD200 (11, 12). The cytoplasmic domain of CD200 R1 contains two non-ITIM tyrosine residues which are required for propagating its inhibitory signals (13-15). CD200 R-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12 (4, 16).

Item	Value
References	Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483. Barclay, A.N. et al. (2002) Trends Immunol. 23:285. Wright, G.J. et al. (2003) J. Immunol. 171:3034. Shiratori, I. et al. (2005) J. Immunol. 175:4441. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348. Fallarino, F. et al. (2004) J. Immunol. 173:3748. Wright, G.J. et al. (2001) Immunology 102:173. Hoek, R.M. et al. (2000) Science 290:1768. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688. Hatherley, D. et al. (2005) J. Immunol. 175:2469. Gorczynski, R. et al. (2004) J. Immunol. 172:7744. Zhang, S. and J.H. Phillips (2006) J. Leukoc. Biol. 79:363. Zhang, S. et al. (2004) J. Immunol. 173:6786. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191. Voehringer, D. et al. (2004) J. Biol. Chem. 279:54117.
Long Name	CD200 Receptor 1
Entrez Gene IDs	131450 (Human); 57781 (Mouse); 102139962 (Cynomolgus Monkey)
Alternate Names	CD200 R1, CD200 receptor 1, CD200R1, CD200RMOX2Rcell surface glycoprotein CD200 receptor 1, CRTR2, Cell surface glycoprotein OX2 receptor 1, HCRTR2, MOX2 receptor, MOX2R, OX2RCD200 cell surface glycoprotein receptor, cell surface glycoprotein receptor CD200

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Recombinant Mouse CD200R1 His-tag Protein, CF

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