Recombinant Mouse CCL5/RANTES Protein

Name: Recombinant Mouse CCL5/RANTES Protein
Brand: R&D Systems
SKU: 478-MR
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 478-MR

Citations (18)

(2)

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Key Product Details

Source:	E. coli
Accession #:	Q5XZF2
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse CCL5/RANTES Protein

Source	E. coli-derived mouse CCL5/RANTES protein Ser24-Ser91
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser24
Predicted Molecular Mass	7.9 kDa
Activity	Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED₅₀ for this effect is 1.5‑9 ng/mL. Measured by its ability to chemoattract 2-day cultured human monocytes. The ED₅₀ for this effect is 30‑100 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

478-MR

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

478-MR/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL5/RANTES

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1 - 3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature mouse CCL5 shares 100% aa sequence identity with rat CCL5 and 75% - 88% with canine, cotton rat, feline, and human CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11 - 13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14 - 16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17).

Item	Value
References	Schall, T.J. et al. (1990) Nature 347:669. Bacon, K.B. et al. (1995) Science 269:1727. Fischer, F.R. et al. (2001) J. Immunol. 167:1637. Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477. Heeger, P. et al. (1992) Kidney Int. 41:220. Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83. Levy, J.A. (2009) J. Immunol. 182:3945. Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37. DeVico, A.L. and Gallo, R.C. (2004) Nat. Rev. Microbiol. 2:401. Proost, P. et al. (1998) J. Biol. Chem. 273:7222. Appay, V. et al. (1999) J. Biol. Chem. 274:27505. Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. 100:1885. von Hundelshausen, P. et al. (2005) Blood 105:924. von Hundelshausen, P. et al. (2001) Circulation 103:1772. Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897. Baltus, T. et al. (2003) Blood 102:1985. Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.
Entrez Gene IDs	6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names	CCL5, D17S136Enormally T-expressed, and presumably secreted, EoCP, Eosinophil chemotactic cytokine, RANTES, SIS-delta, SISd, Small-inducible cytokine A5, T cell-specific protein P228, T-cell specific protein p288, TCP228T-cell-specific protein RANTES, chemokine (C-C motif) ligand 5, small inducible cytokine A5 (RANTES), small inducible cytokine subfamily A (Cys-Cys), member 5

Citations for Recombinant Mouse CCL5/RANTES Protein (18)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse CCL5/RANTES Protein include:

Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Y Lin et al. (2021), Neddylation pathway alleviates chronic pancreatitis by reducing HIF1&alpha-CCL5-dependent macrophage infiltration Cell Death Disease, 2021;12(3):273.
PMID: 33723230
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

MH Ho et al. (2021), CCL5 via GPX1 activation protects hippocampal memory function after mild traumatic brain injury Redox Biology, 46(0):102067.
PMID: 34315111
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

R Ajoy et al. (2021), CCL5 promotion of bioenergy metabolism is crucial for hippocampal synapse complex and memory formation Molecular Psychiatry, 0(0).
PMID: 33931731
Species: Mouse

Applications: Binding Assay

S Sharif et al. (2019), Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity Front Microbiol, 10(0):1421.
PMID: 31293551
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

JP Böttcher et al. (2018), NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control Cell, 172(5):1022-1037.e14.
PMID: 29429633
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

JW Lee et al. (2017), The HIV co-receptor CCR5 regulates osteoclast function Nat Commun, 8(1):2226.
PMID: 29263385
Species: Mouse

Applications: In Vivo

N Suffee et al. (2017), Pro-angiogenic effect of RANTES-loaded polysaccharide-based microparticles for a mouse ischemia therapy Sci Rep, 7(1):13294.
PMID: 29038476
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

SW Huang et al. (2017), P2X7 receptor-dependent tuning of gut epithelial responses to infection Immunol. Cell Biol, 95(2):178-188.
PMID: 27559003
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Pan et al. (2017), Ccl5 establishes an autocrine high-grade glioma growth regulatory circuit critical for mesenchymal glioblastoma survival Oncotarget, 0(0).
PMID: 28380429
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Costa M et al. (2015), Murine IL-17+ Vgamma4 T lymphocytes accumulate in the lungs and play a protective role during severe sepsis. BMC Immunol, 16(0):36.
PMID: 26037291
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Sottnik J et al. (2015), Tumor-induced pressure in the bone microenvironment causes osteocytes to promote the growth of prostate cancer bone metastases. Cancer Res, 75(11):2151-8.
PMID: 25855383
Species: Mouse
Sample Types: Cell Lysates
Applications: ELISA (Standard)

Yang L et al. (2015), KIM-1-mediated phagocytosis reduces acute injury to the kidney. J Clin Invest, 125(4):1620-36.
PMID: 25751064
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Sato S et al. (2014), The Friend leukaemia virus integration 1 (Fli-1) transcription factor affects lupus nephritis development by regulating inflammatory cell infiltration into the kidney. Clin Exp Immunol, 177(1):102-9.
PMID: 24580413
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Kimura et al. (2014), The role of CCL5 in the ability of adipose tissue-derived mesenchymal stem cells to support repair of ischemic regions. Stem Cells Dev, 23(5):488-501.
PMID: 24171667
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Wintges K et al. (2013), Impaired bone formation and increased osteoclastogenesis in mice lacking chemokine (C-C motif) ligand 5 (Ccl5). J Bone Miner Res, 28(10):2070-80.
PMID: 23553711
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Kim J et al. (2011), Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines. Am. J. Pathol., 179(6):2730-9.
PMID: 21967814
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Binder NB et al. (2009), Estrogen-dependent and C-C chemokine receptor-2-dependent pathways determine osteoclast behavior in osteoporosis. Nat. Med., 15(4):417-24.
PMID: 19330010
Chou SY et al. (2008), Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes. J. Neurosci., 28(13):3277-90.
PMID: 18367595

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Customer Reviews for Recombinant Mouse CCL5/RANTES Protein (2)

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Recombinant Mouse CCL5/RANTES Protein

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Application: Immunoassay Standard

Verified Customer | Posted 08/12/2019
Recombinant Mouse CCL5/RANTES Protein

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 11/27/2017

The recombinant protein was used as an ELISA calibrator employing MAB478 and BAF478 as the matched pair antibodies.

Recombinant Mouse CCL5/RANTES Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Mouse CCL5/RANTES Protein

Formulation, Preparation and Storage

Carrier Free

478-MR

Carrier

478-MR/CF

Carrier Free

Reconstitution Calculator

Background: CCL5/RANTES

Citations for Recombinant Mouse CCL5/RANTES Protein (18)

Customer Reviews for Recombinant Mouse CCL5/RANTES Protein (2)

Customer Images

Recombinant Mouse CCL5/RANTES Protein

Recombinant Mouse CCL5/RANTES Protein

Product Documents for Recombinant Mouse CCL5/RANTES Protein

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