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Recombinant Mouse Cadherin-13 (CAD-13) Protein, CF

Bio-Techne includes R&D Systems | Catalog # 6768-CA

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6768-CA-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Cadherin-13 protein
Met1-Gly693, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala22

Predicted Molecular Mass

74.4 kDa

SDS-PAGE

100-120 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to inhibit the adhesion of HUVEC human umbilical vein endothelial cells.
When 5 x 104 cells/well are added to Recombinant Human Cadherin-13 (Catalog # 3264-CA) coated plates, cell adhesion is inhibited in a dose dependent manner after 2.5 hours at 37 °C. The ED50 for this effect is 1.0‑5.0 μg/mL.

Formulation, Preparation and Storage

6768-CA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cadherin-13

Cadherin-13, also known as T-Cadherin and H‑Cadherin, is a GPI-anchored protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Following removal of its C‑terminal propeptide, Cadherin-13 is expressed on the cell surface both with and without its N-terminal propeptide in species of approximately 120 kDa and 100 kDa, respectively (3, 4). Mouse Cadherin-13 contains five tandem Cadherin repeats and shares 95% and 98% aa sequence identity with human and rat Cadherin-13, respectively (5). It is most highly expressed in the circulatory and nervous systems, particularly on cardiac myocytes, vascular endothelial and smooth muscle cells, and neurons and astrocytes in the brain (3, 4, 6 - 9). Cadherin-13 exerts a negative influence on neurite extension, and it is down‑regulated in neurons upon NGF stimulation (10, 11). Homotypic Cadherin-13 interactions promote intercellular adhesion which can be inhibited by its binding to LDL (12). Cadherin-13 also mediates the cardioprotective effects of Adiponectin by directly binding Adiponectin (selectively the hexameric and HMW forms) and trapping it on vascular endothelial cells and cardiomyocytes (6 ‑ 8). The role of Cadherin-13 in the vasculature is complex. When expressed on vascular endothelial cells it promotes angiogenesis, but when expressed on stromal cells it inhibits neovascularization (13, 14). Its down‑regulation on breast carcinoma cells and up‑regulation on the vasculature of various tumors limits tumor cell proliferation and angiogenesis but also enhances tumor progression (4, 8, 15).

References

  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Philippova, M. et al. (2009) Cell. Signal. 21:1035.
  3. Stambolsky, D.V. et al. (1999) Biochim. Biophys. Acta 1416:155.
  4. Doyle, D.D. et al. (1998) J. Biol. Chem. 273:6937.
  5. Wyder, L. et al. (2000) Cancer Res. 60:4682.
  6. Denzel, M.S. et al. (2010) J. Clin. Invest. 120:4342.
  7. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
  8. Hebbard, L.W. et al. (2008) Cancer Res. 68:1407.
  9. Takeuchi, T. et al. (2000) J. Neurochem. 74:1489.
  10. Fredette, B.J. et al. (1996) Development 122:3163.
  11. Bai, S. et al. (2007) J. Biol. Chem. 282:27171.
  12. Resink, T.J. et al. (1999) FEBS Lett. 463:29.
  13. Philippova, M. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26:2222.
  14. Rubina, K. et al. (2007) Angiogenesis 10:183.
  15. Lee, S.W. (1996) Nat. Med. 2:776.

Alternate Names

Cadherin13, CDH13, H-Cadherin, T-Cadherin

Entrez Gene IDs

1012 (Human); 12554 (Mouse); 192248 (Rat)

Gene Symbol

CDH13

UniProt

Additional Cadherin-13 Products

Product Documents for Recombinant Mouse Cadherin-13 (CAD-13) Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Cadherin-13 (CAD-13) Protein, CF

For research use only

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