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Recombinant Mouse BTNL3 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10222-BT

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10222-BT-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse BTNL3 protein
Recombinant Mouse BTNL3
(Glu28-Pro241)
Accession # Q7TST0.3
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu28

Predicted Molecular Mass

51 kDa

SDS-PAGE

45-65 kDa, under reducing conditions

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3.
The ED50 for this effect is 0.4-4 μg/mL.

Scientific Data Images for Recombinant Mouse BTNL3 Fc Chimera Protein, CF

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.4-4 μg/mL.
2 μg/lane of Recombinant Mouse BTNL3 Fc Chimera (Catalog # 10222-BT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-65 kDa and 90-130 kDa, respectively.

Formulation, Preparation and Storage

10222-BT
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: BTNL3

Butyrophilin-like 3 (BTNL3), also referred to as BTNL1, Gm316, and Ng10, is a member of the BTN/MOG Ig-superfamily and shares structural resemblance to the B7 family (1). BTNL3 is a type I transmembrane protein that consists of an extracellular domain (ECD) containing two IgV domains, a single transmembrane domain, and a cytoplasmic domain with a B30.2/SPRY region. Mature mouse BTNL3 shares 30% and 80% amino acid sequence identity with human and rat BTNL3, respectively. BTNL3 mRNA has been identified in many tissues including small intestine, colon, liver, lung, bone marrow and spleen tissue, and it is highly expressed in neutrophils (2-6). The specific function of BTNL3 has yet to be fully elucidated, but BTNL3 is suggested to be a novel negative regulator for T cell activation and immune diseases (7). In humans, BTNL3 has been shown to form heterodimers with BTNL8 and overexpression of this complex leads to CD69 upregulation and T cell antigen receptor downregulation (8). In mice, BTNL3 can form heterodimers with BTNL6 and modulate local immune responses and intraepithelial lymphocytes–epithelial cell interaction pathways (9). Additionally, BTNL3 has been shown to bind directly to V gamma4+ T cell receptor, suggesting a role in gamma delta T cell regulation (10). BTNL3 expression has also been found to be down-regulated in colon cancer tumors (11). R&D Systems in house data indicate that mouse BTNL3 protein inhibits the secretion of IL-2 from anti-CD3 activated mouse CD3+ T cells.

References

  1. Shibui, A. et al. (1999) J. Hum. Genet. 44:249.
  2. Arnett, H.A. et al. (2014) Nat Rev Immunol. 14:5596.
  3. Arnett, H.A. et al. (2009) Cytokine 46:370.
  4. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  5. Rhodes, D. et al. (2016) Annu. Rev. Immunol. 34:151.
  6. Guo, Y and Wang, AY (2015) Front. Immunol. 6:421.
  7. Yamazaki, T. et al. (2010) J Immunol 185:5907.
  8. Melandri, D. et al. (2018) Nat Immunol 19:1352.
  9. Lebrero-Fernández, C. et al. (2016) Front Immunol. 7:1.
  10. Wilcox, C.R. et al. (2019) Immunity 51:813.
  11. Lebrero-Fernandez, C. et al. (2016) Immun. Inflammation Dis. 4:191.

Long Name

Butyrophilin-like 3

Alternate Names

BTN9.1, BTNLR, COLF4100

Entrez Gene IDs

10917 (Human); 100038862 (Mouse); 407781 (Rat)

Gene Symbol

BTNL3

UniProt

Additional BTNL3 Products

Product Documents for Recombinant Mouse BTNL3 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse BTNL3 Fc Chimera Protein, CF

For research use only

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