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Recombinant Mouse Active Cathepsin C/DPPI Protein, CF

Bio-Techne includes R&D Systems | Catalog # 2336-CY

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2336-CY-010

Key Product Details

Source

NS0

Accession #

Structure / Form

Active form

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Cathepsin C/DPPI protein
proform (Asp25-Leu462 with a C-terminal 10-His tag)
The proform was activated by Recombinant Human Cathepsin L (Catalog # 952-CY) and further purified.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp25 (Exclusion domain) and Asp394 (Light chain)

Predicted Molecular Mass

13 kDa (Exclusion domain), 18 kDa (Heavy chain), and 9 kDa (Light chain)

SDS-PAGE

8 kDa and 20-27 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Arg-7-amido-4-methylcoumarin (GR-AMC).
The specific activity is > 60,000 pmol/min/µg, as measured under the described conditions. 

Formulation, Preparation and Storage

2336-CY
Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Cathepsin C/DPPI

Cathepsin C (CTSC) is a cysteine protease of the papain family (1). It sequentially removes dipeptides from the free N-termini of proteins and peptides. It has broad specificity except that it does not cleave a basic amino acid (Arg or Lys) in the N-terminal position or Pro on either side of the scissle bond. It requires halide ions for activity. The pro form contains a pro region and a mature region, which are further cleaved during activation to remove the prodomain and split the catalytic domain into heavy and light chains. The N-terminal domain of the pro region is also called the exclusion domain, which remains connected to the heavy chain through non-covalent bonds to the mature, active enzyme. Broadly distributed, CTSC plays a major role in lysosomal degradation and enzyme activation. For example, it activates granule serine proteases in cytotoxic T lymphocytes and natural killer cells (granzymes A and B), mast cells (tryptase and chymase), and neutrophils (Cathepsin G and elastase) by removing their N-terminal activation dipeptides (2).

References

  1. Turk, et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 1192, Academic Press, San Diego.
  2. Dahl, et al. (2001) Biochemistry 40:1671.

Alternate Names

CTSC, DPPI, PALS, PLS

Entrez Gene IDs

1075 (Human); 13032 (Mouse); 25423 (Rat)

Gene Symbol

CTSC

UniProt

Additional Cathepsin C/DPPI Products

Product Documents for Recombinant Mouse Active Cathepsin C/DPPI Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Active Cathepsin C/DPPI Protein, CF

For research use only

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