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Recombinant Human Tryptase gamma-1/TPSG1 Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1667-SE

Catalog #
Size / Price

Key Product Details



Accession #

Structure / Form

Pro form




Enzyme Activity

Product Specifications


Mouse myeloma cell line, NS0-derived human Tryptase gamma-1/TPSG1 protein
Arg20-Arg281, with a C-terminal 10-His tag


>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis


Predicted Molecular Mass

29 kDa


36 kDa, reducing conditions


Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >70 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Formulation Supplied as a 0.2 μm filtered solution in Tris, CaCl2, NaCl, Brij and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Tryptase gamma-1/TPSG1

As mediators of inflammatory and allergic response, mast cells are found throughout the body concentrated near blood vessels in connective tissue and the mucous membranes of the respiratory and gastrointestinal tract (3). Upon activation, human mast cells release granules that are enriched with neutral serine proteases including Tryptases, chymase and cathepsin G (4). Tryptase gamma-1, also called transmembrane Tryptase, is encoded by TPSG1, one of many serine protease genes clustered in human chromosome 16p13.3 (5). Human Tryptase gamma-1 is synthesized as a 321 amino acid preproenzyme with a C-terminal transmembrane anchor (1, 2). The rhTPSG1 was expressed as a soluble protein terminated at residue 281 and corresponded to the proenzyme. The proenzyme can be cleaved by trypsin to form the active enzyme. The serine protease activity of trypsin-activated rhTPSG1 can be inhibited by ecotin (R&D Systems, Catalog # 1328-PI). Greater than 95% protease activity is inhibited by ecotin at approximately 10:1 molar ratio.


  1. Wong, G.W. et al. (1999) J. Biol. Chem. 274:30784.
  2. Caughey, G.H. et al. (2000) J. Immunol. 164:6566.
  3. Harris, J.L. et al. (2001) J. Biol. Chem. 276:34941.
  4. Miller, H.R.P. and A.D. Pemberton (2002) Immunology 105:375.
  5. Wong, G.W. et al. (2002) J. Biol. Chem. 277:41906.

Alternate Names

TPSG1, Tryptase gamma1

Entrez Gene IDs

25823 (Human)

Gene Symbol



Additional Tryptase gamma-1/TPSG1 Products

Product Documents for Recombinant Human Tryptase gamma-1/TPSG1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Tryptase gamma-1/TPSG1 Protein, CF

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