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Recombinant Human TIGAR/C12orf5 His-tag Protein, CF

Catalog # 11008-TG | R&D Systems, Inc. a Bio-Techne Brand
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11008-TG-020

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

E. coli-derived human TIGAR/C12orf5 protein
Ala2-Arg270 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2

Predicted Molecular Mass

31 kDa

SDS-PAGE

27-30 kDa, under reducing conditions

Activity

Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP).
The specific activity is >10 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human TIGAR/C12orf5 His-tag Protein, CF

Recombinant Human TIGAR/C12orf5 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human TIGAR/C12orf5 His-tag Protein (Catalog # 11008-TG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 27-30 kDa.

Formulation, Preparation and Storage

11008-TG
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: TIGAR/C12orf5

Fructose-2,6-bisphosphatase TIGAR (TIGAR), also known as TP53-induced glycolysis and apoptosis regulator, is a highly conserved, ubiquitously expressed enzyme from the phosphoglycerate mutase family that hydrolyzes fructose-2,6-bisphosphate, a key regulator of cellular metabolism that activates glycolysis and inhibits gluconeogenesis (1). Consequently, TIGAR acts as a negative regulator of glycolysis and its activity results in pentose phosphate pathway activation and NADPH production. Human TIGAR is a 270 amino acid monomeric protein with a histidine phosphatase fold containing a phosphate coordinated to a catalytic histidine in the active site (2). It is located both in the cytoplasm and in organelles where its function may vary (1); translocation of TIGAR to the nucleus promotes cell survival during chemotherapy and hypoxia (3) while transportation to the mitochondria under hypoxia can enhance HK2 activity and maintain cell survival (4) or interact with ATP5A1 to reduce oxidative stress (1). TIGAR is a target gene of p53 through binding sequences in the promoter region (5) and may promote cancer metastasis through both enzymatic function and through interactions that allow it to mediate signaling pathways that inhibit autophagy and apoptosis (6, 7). TIGAR is highly expressed in some tumors (1) and may be a potential therapeutic target for cancers (6-8), cardiovascular disease (9), and neurological diseases (10-13).

References

  1. Tang, J. et al. (2021) Acta Pharmacol. Sin. 42:1547.
  2. Li, H. and G. Jogl (2009) J Biol. Chem. 284:1748.
  3. Yu, H.P. et al. (2015) Sci. Rep. 5:9853.
  4. Cheung, E.C. et al. (2012) Proc. Natl. Acad. Sci. USA 109:20491.
  5. Bensaad, K. et al. (2006) Cell 126:107.
  6. Bartrons, R. et al. (2018) Front Oncol. 8:331.
  7. Li, L. et al. (2021) Oxid. Med. Cell Longev. 2021:8877460.
  8. Chandel, V et al. (2021) 3 Biotech. 11:117.
  9. Zhao, Z.W. et al. (2021) Atherosclerosis 327:76.
  10. Chen, J. et al. (2018) Neuropharmacology 131:377.
  11. Tang, Z. et al. (2019) Oncol. Lett. 18:2509.
  12. Li, Q.Q. et al. (2021) Neurochem. Int. 148:105081.
  13. Lei, B. et al. (2021) Front Cell Neurosci. 15:653881.

Long Name

TP53-induced Glycolysis and Apoptosis Regulator

Alternate Names

C12orf5

Entrez Gene IDs

57103 (Human); 319801 (Mouse); 502894 (Rat)

Gene Symbol

TIGAR

UniProt

Additional TIGAR/C12orf5 Products

Product Documents for Recombinant Human TIGAR/C12orf5 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human TIGAR/C12orf5 His-tag Protein, CF

For research use only

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