Recombinant Human TGF-beta RII Isoform 2 Fc Chimera, CF

Name: Recombinant Human TGF-beta RII Isoform 2 Fc Chimera, CF
Brand: R&D Systems
SKU: 1003-RT

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1003-RT

Citations (3)

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	NP_001020018
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human TGF-beta RII Isoform 2 Fc Chimera, CF

Source

Mouse myeloma cell line, NS0-derived human TGF-beta RII protein

Human TGFâ€‘ beta RII Isoform 2 (Thr23-Asp184) & (Ile24-Asp184) Accession # NP_001020018	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Thr23 & Ile24

Predicted Molecular Mass

45 kDa (monomer)

SDS-PAGE

65-75 kDa, reducing conditions

Activity

Measured by its ability to inhibit TGF-beta 1 activity on HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED₅₀ for this effect is 1-3 ng/mL in the presence of 0.1 ng/mL of recombinant human TGF-beta 1.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1003-RT

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta RII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70‑85 kDa) and Type III (250‑350 kDa). The Type III receptor, a proteoglycan that exists in membrane‑bound and soluble forms, binds TGF-beta 1, TGF-beta 2 and TGF-beta 3, and appears to participate in both TGF-beta dependent and independent cellular signaling. The Type II receptor, by contrast, is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity, and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that requires the presence of the Type II receptor to bind TGF-beta. Evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors. TGF-beta receptor II isoform 2 (also TGF-beta RII isoform A) is an alternatively spliced variant of the standard Type II TGF-beta receptor (or TGF-beta RII isoform 1) that possesses a 27 amino acid substitution for Val10 near the N-terminus of the mature protein. Both T beta RII and T beta RII isoform 2 bind TGF-beta 1 and TGF-beta 3 with high affinity. However, only TGF-beta RII isoform 2 also binds TGF-beta 2 with high affinity in the absence of TGF‑ beta RIII. While TGF-beta RII is widely expressed on cells, TGF-beta RII isoform 2 shows distinct expression pattern mainly associated with bone‑related cells, such as osteoblasts and mesenchymal precursor cells. TGF-beta RII isoform 2 may play an important role in TGF-beta 2 binding and signaling in cells lacking TGF-beta RIII.

Item	Value
Long Name	Transforming Growth Factor beta Receptor II
Entrez Gene IDs	7048 (Human); 21813 (Mouse)
Alternate Names	AAT3, EC 2.7.11, EC 2.7.11.30, FAA3, HNPCC6, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TGF-bRII, TGF-beta RII, TGF-beta receptor type II, TGF-beta receptor type IIB, TGF-beta receptor type-2, TGF-beta type II receptor, TGFBR2, TGFR-2, TGFbeta-RII, TGFbetaRII, Transforming growth factor-beta receptor type II, tbetaR-II, transforming growth factor beta receptor type IIC, transforming growth factor, beta receptor II (70-80kD), transforming growth factor, beta receptor II (70/80kDa), transforming growth factor, beta receptor II (70/80kDa) isoform 1, transforming growth factor, beta receptor II (70/80kDa) isoform 2

Citations for Recombinant Human TGF-beta RII Isoform 2 Fc Chimera, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human TGF-beta RII Isoform 2 Fc Chimera, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

OE Olsen et al. (2017), TGF-? contamination of purified recombinant GDF15 PLoS ONE, 12(11):e0187349.
PMID: 29161287
Species: Bacteria
Sample Types: Trypanosoma
Applications: PcIP

Lu B et al. (2008), A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi. Infect. Immun., 76(8):3530-8.
PMID: 18541656
Species: Human
Sample Types: Cell Culture Supernates
Applications: Bioassay

Godfrey WR et al. (2005), Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Blood, 105(2):750-8.
PMID: 15374887

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