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Recombinant Human TfR2 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10486-TF

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10486-TF-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TfR2 protein
MD Human IgG1
(Pro100-Lys330)
IEGR Human TfR2
(Gly112-Phe801)
Accession # NP_003218.2
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

103 kDa

SDS-PAGE

105-117 kDa, under reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Human TfR2 Fc Chimera Protein, CF

Recombinant Human TfR2 Fc Chimera Protein SDS-PAGE

Recombinant Human TfR2 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human TfR2 Fc Chimera Protein (Catalog # 10486-TF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-117 kDa and 180-210 kDa, respectively.

Formulation, Preparation and Storage

10486-TF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TfR2

Transferrin Receptor 2 (TfR2 or TfR2 alpha) is an iron-binding type 2 transmembrane glycoprotein homologous to the Transferrin Receptor 1 (TfR or TfR1) (1). It forms a 215 kDa disulfide-linked homodimer and is predominantly expressed in the liver and modulates hepcidin production in response to iron (1, 2). Human TfR2 cDNA encodes 801 amino acids (aa) including an 83 aa N-terminal intracellular region, a 21 aa transmembrane domain, and a 697 aa extracellular domain (ECD). Human TfR2 ECD shares 86% and 45% aa identity with mouse TfR2 and human TfR1, respectively. An alternative splicing isoform (TfR2 beta) lacks the intracellular region, transmembrane domain and part of the extracellular regions and is expressed ubiquitously (1, 3). The Iron loaded Transferrin (holo-Transferrin) circulating in blood binds to cell-surface receptor TFR2 (4). Mutations in TFR2 can cause a form of hereditary hemochromatosis (HH, type 111), a disorder marked by chronic iron overload (5). 

References

  1. Kawabata, H. et al. (1999) J. Biol. Chem. 274:20826.
  2. Trinder, D. and E. Baker (2003) Int. J. Biochem. Cell. Biol. 35:292.
  3. Deaglio, S. et al. (2002) Blood 100:3782.
  4. Aisen, P. et al. (1978) J. Biol Chem. 253:1930.
  5. Camaschella, C. et al. (2000) Nat Genet. 25:14.

Long Name

Transferrin Receptor 2

Alternate Names

HFE3, TFRC2

Entrez Gene IDs

7036 (Human); 50765 (Mouse)

Gene Symbol

TFR2

UniProt

Additional TfR2 Products

Product Documents for Recombinant Human TfR2 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human TfR2 Fc Chimera Protein, CF

For research use only

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