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Recombinant Human SCUBE3 Protein, CF

Catalog # 7730-SC | R&D Systems, Inc. a Bio-Techne Brand
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7730-SC-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human SCUBE3 protein
Ala21-Lys993, with an N-terminal HA-tag (YPYDVPDYA)

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr & Arg538

Predicted Molecular Mass

108.3 kDa (Monomer)

SDS-PAGE

110-130 kDa & 60-70 kDa, reducing conditions  

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is typically 0.25-1.25 μg/mL.

Formulation, Preparation and Storage

7730-SC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCUBE3

SCUBE3 (signal peptide, CUB and EGF-like domain-containing protein 3) is a member of the SCUBE family of secreted glycoproteins (1). Like other SCUBE proteins, the 993 amino acid (aa) human SCUBE3 precursor contains a signal peptide (aa 1‑20), nine EGF‑like motifs (aa 29‑398), a linker region with multiple N‑glycosylation sites (aa 399‑803) and a C‑terminal CUB domain (aa 804‑916). Cysteines within the EGF‑like and linker regions are mainly conserved (1). The 130 kDa, mature, full‑length form of human SCUBE3 shares 96%, 93%, 96%, 95%, 95% and 94% aa sequence identity with mouse, rat, equine, bovine, canine and porcine SCUBE3, respectively (1). It also shares 66% and 58% aa identity with human SCUBE1 and SCUBE2, respectively. Cleavage by proteases such as MMP‑2 and MMP‑9 produces a CUB domain-containing, 55 kDa fragment that, like full‑length SCUBE3, binds TGF-beta RII (1, 2). SCUBE proteins form homo-oligomers and hetero‑oligomers, and are predicted to affect TGF‑ beta and Hedgehog signaling (2‑5). SCUBE3 is most highly expressed in osteoblasts (1, 3, 6). It is also found in umbilical vein endothelial cells with SCUBE1 and SCUBE2, and in cardiac ventricular myocytes (1, 3, 6). Transgenic over-expression of SCUBE3 can cause progressive cardiac hypertrophy (3). SCUBE3 and its active fragment are frequently over‑expressed in lung cancer tumor tissues, and can promote epithelial to mesenchymal transition by enhancing TGF-beta signaling pathways (2).

References

  1. Wu, B.T. et al. (2004) J. Biol. Chem. 279:37485.
  2. Wu, Y.Y. et al. (2011) Oncogene 30:3682.
  3. Yang, H.Y. et al. (2007) Cardiovasc. Res. 75:139.
  4. Johnson, J.L. et al. (2012) Dev. Biol. 368:193.
  5. Creanga, A. et al. (2012) Genes Dev. 26:1312.
  6. Haworth, K. et al. (2007) Gene Expr. Patterns 7:630.

Alternate Names

CEGF3

Entrez Gene IDs

222663 (Human); 268935 (Mouse); 294297 (Rat)

Gene Symbol

SCUBE3

UniProt

Product Documents for Recombinant Human SCUBE3 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human SCUBE3 Protein, CF

For research use only

Reconstitution Calculator

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