Skip to main content

Novus Biologicals products are now on

Better as one

Recombinant Human S100B Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1820-SB

Catalog #
Size / Price

Key Product Details


E. coli

Accession #

Structure / Form






Product Specifications


E. coli-derived human S100B protein


>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis


Predicted Molecular Mass

10.7 kDa


7 kDa, reducing conditions


Measured in a cell proliferation assay using human peripheral blood monocytes.
The ED50 for this effect is 1-4 μg/mL.

Formulation, Preparation and Storage

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100B

S100B, previously called S100 beta, belongs to the S100 family within the EF-hand superfamily of Ca2+ binding proteins (1-3). S100 proteins contain two EF-hand motifs that differ in affinity, separated by a hinge region with a hydrophobic cleft that is exposed upon Ca2+ binding (4). S100B is a 91 amino acid (aa) protein, after removal of the initial methionine, and is found as homodimers of 10.4 kDa monomers. Human S100B shares 99%, 98%, 100%, 99% and 97% aa sequence identity with mouse, rat, rabbit, equine and bovine S100B, respectively. Within the S100 family, human S100B shows the highest aa identity (59%) with S100A1. S100B is expressed primarily by astrocytes and oligodendrocytes in the central nervous system, and by Schwann cells in the peripheral nervous system (5). Ca2+-bound S100B interacts in vitro with at least 20 cytoplasmic proteins, including several structural molecules such as tubulin and GFAP (1, 4). It can inhibit the phosphorylation of these kinase substrates and others such as tau and neuromodulin (1, 5). Astrocytes can secrete S100B, which then acts in a cytokine-like manner (5, 6). Nanomolar concentrations of S100B are secreted constitutively, promote proliferation, and are neurotrophic and anti-apoptotic (1, 6). Blood levels of S100B reflect extracellular concentrations within the nervous system, and are elevated in Down’s syndrome, Alzheimer’s disease and Tourette’s syndrome, metabolic stress, acute brain injury and brain tumors (5, 7). Micromolar concentrations of S100B can be destructive and pro-apoptotic; they induce the expression of iNOS, COX-2, IL-1, IL‑6 and TNF-alpha by microglia, astrocytes or neurons (6, 8-11). Most extracellular actions of S100B can be mediated by RAGE (receptor for advanced glycation end products), which is also a receptor for other S100 proteins (6, 10, 11).


  1. Michetti, F. et al. (2012) J. Neurochem. 120:644.
  2. Marenholz, I. et al. (2004) Biochem. Biophys. Res. Commun. 322:1111.
  3. Allore, R.J. et al. (1990) J. Biol. Chem. 265:15537.
  4. McClintock, K.A. and G.S. Shaw (2003) J. Biol. Chem. 278:6251.
  5. Sen, J. & A. Belli (2007) J. Neurosci. Res. 85:1373.
  6. Huttunen, H.J. et al. (2000) J. Biol. Chem. 275:40096.
  7. Gerlach, R. et al. (2006) Neuroscience 141:1697.
  8. Hu, J. et al. (1996) J. Biol. Chem. 271:2543.
  9. Liu, L. et al. (2005) J. Neurochem. 92:546.
  10. Ponath, G. et al. (2007) J. Neuroimmunol. 184:214.
  11. Bianchi, R. et al. (2007) J. Leukoc. Biol. 81:108.

Long Name

S100 Calcium Binding Protein B

Alternate Names

beta (neural), NEF, S100, S100 beta, S100 calcium binding protein B, S100 calcium-binding protein B, S100 calcium-binding protein, beta (neural), S-100 calcium-binding protein, beta chain, 10protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100beta

Entrez Gene IDs

6285 (Human)

Gene Symbol



Additional S100B Products

Product Documents for Recombinant Human S100B Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human S100B Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.