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Recombinant Human RGM-A Protein, CF

Catalog # 2459-RM | R&D Systems, Inc. a Bio-Techne Brand
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2459-RM-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human RGM-A protein
Cys48-Gly422 & Pro169-Gly422, both with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys48 & Pro169

Predicted Molecular Mass

42.4 kDa

SDS-PAGE

Multiple bands between 25-37 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When rhBMP-4 is coated at 1 μg/mL, rhRGM-A binds with an apparent KD <50nM.

Immobilized rhRGM-A can bind rmNeogenin/Fc Chimera with a linear range of
10-500 ng/mL.

Formulation, Preparation and Storage

2459-RM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RGM-A

Human Repulsive Guidance molecule (RGM) is a 33 kDa GPI-linked member of an expanding RGM-related family of neuronal and muscle-expressed membrane proteins (1). It is synthesized as a 450 amino acid (aa) preproprotein that contains a 47 aa signal sequence, a 121 aa N-terminal prosegment, a 256 mature region and a 26 aa C-terminal prosegment (2). The N-terminal prosegment contains an RGD tripeptide and the molecule’s only two potential N-linked glycosylation sites. The mature segment shows an abbreviated von Willebrand factor domain. Proteolytic processing occurs at an aspartic acid-proline bond, creating a predicted 32 kDa mature region (2). The mature region of human RGM-A has 88% and 93% aa identity to the chick and mouse mature region of RGM-A, respectively. When compared to human RGMb and c, the mature region of human RGM-A shows 58% and 54% aa identity, respectively. Recombinant chick RGM-A has been reported to induce collapse of temporal but not nasal growth cones, and to repel temporal retinal axons in vitro. This suggests a role in the development of the retina-superior colliculus connection. In mammals, however, this activity is not so evident, and thus its function in this system is uncertain (3). Alternatively, in mice, RGM-A is said to be needed for neural tube closure, and may play a role in entorhinal-hippocampal connections (3, 4). The receptor for RGM-A is reported to be neogenin (5, 6). RGM-A has also been shown to be a bone morphogenic protein co-receptor, able to bind both BMP-2 and BMP-4 (7).

References

  1. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
  2. Monnier P. et al. (2002) Nature 419:392.
  3. Niederkofler V. et al. (2004) J. Neurosci. 24:808.
  4. Brinks, H. et al. (2004) J. Neurosci. 24:3862.
  5. Rajagopalan S. et al. (2004) Nat. Cell Biol. 6:756.
  6. Matsunaga E. et al. (2004) Nat. Cell Biol. 6:749.
  7. Babitt J.L. et al. (2005) J. Biol. Chem. 280(33):29820.

Long Name

Repulsive Guidance Molecule A

Alternate Names

RGMA

Entrez Gene IDs

56963 (Human); 244058 (Mouse)

Gene Symbol

RGMA

UniProt

Product Documents for Recombinant Human RGM-A Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human RGM-A Protein, CF

For research use only

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