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Recombinant Human RELT/TNFRSF19L Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1385-RT

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1385-RT-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human RELT/TNFRSF19L protein
Human RELT
Ser26-Ala160 (Arg127Gly and Arg129Gly)
Accession #Q969Z4
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser26

Predicted Molecular Mass

41 kDa (monomer)

SDS-PAGE

55-58 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

1385-RT
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: RELT/TNFRSF19L

RELT (Receptor Expressed in Lymphoid Tissues) is a 46 kDa (predicted) type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily, designated TNFRSF19-like (TNFRSF19L) (1, 2). It is primarily expressed in hematopoietic tissues and peripheral blood leukocytes (2, 3). Human RELT cDNA encodes 430 amino acids (aa), including a 25 aa signal peptide, a 137 aa extracellular domain containing a TNF receptor cysteine-rich domain and a potential N‑linked glycosylation site, a 21 aa transmembrane domain and a 247 aa cytoplasmic region that lacks a death domain. Within the ECD, human RELT shares 78%, 75%, 75%, 78% and 72% aa sequence homology with mouse, rat, canine, porcine and bovine RELT, respectively. Among TNFRSF members, the RELT extracellular domain is most closely related to that of TROY/TNFRSF19 and OX40 (2). Neither human nor mouse RELT bind any of the ~19 TNF superfamily ligands that have been tested (1). Two related transmembrane proteins, RELL1 and RELL2, have been identified in both human and mouse (3). RELL1 and 2 can interact with and are coexpressed with RELT, and are thought to modulate its signaling (3-5). Intracellularly, RELT has been shown to bind the adaptor protein TRAF-1 and activate the NF‑ kappaB pathway, the phospholipid scramblase PLSCR1, and oxidative stress responsive proteins OSR1 (2-5). Another investigator notes association and signaling through SPAK, but not TRAF or NF kappaB (6). When overexpressed in HEK-293 cells, RELT induces p38 and JNK signaling and activates apoptosis (4-6). Immobilized RELT can also costimulate T-cell proliferation in the presence of CD3 signaling (2).

References

  1. Bossen C. et al. (2006) J. Biol. Chem. 281:13964.
  2. Sica, G. et al. (2001) Blood 97:2702.
  3. Cusick, J.K. et al. (2006) Biochem. Biophys. Res. Commun. 340:535.
  4. Cusick, J.K. et al. (2010) Cell. Immunol. 261:1.
  5. Cusick, J.K. et al. (2012) Mol. Cell. Biochem. 362:55.
  6. Polek T.C. et al. (2006) Biochem. Biophys. Res. Commun. 343:125.

Long Name

Receptor Expressed in Lymphoid Tissues

Alternate Names

TNFRSF19L

Entrez Gene IDs

84957 (Human); 320100 (Mouse); 101865743 (Cynomolgus Monkey)

Gene Symbol

RELT

UniProt

Additional RELT/TNFRSF19L Products

Product Documents for Recombinant Human RELT/TNFRSF19L Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human RELT/TNFRSF19L Fc Chimera Protein, CF

For research use only

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