Human embryonic kidney cell, HEK293-derived human PILR-beta protein Gln20-Ala189, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Gln20, as confirmed by mass spectrometry
Predicted Molecular Mass
30-40 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Human CL-P1/COLEC12 (Catalog # 2690-CL) is immobilized at 2 μg/mL (100 μL/well), it binds Recombinant Human PILR‑ beta (Catalog # 10319-PR) with an ED50 of 1-10 μg/mL
Recombinant Human PILR-beta His-tag Protein, CF Scientific Data Examples
2 μg/lane of Recombinant Human PILR-beta His-tag Protein (Catalog # 10319-PR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 30-40 kDa under reducing and non-reducing conditions.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in PBS.
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
3 months, -20 to -70 °C under sterile conditions after opening.
Paired immunoglobulin-like type 2 receptor beta (PILR-beta ) is a type I transmembrane (TM) glycoprotein that belongs to the Ig superfamily (1). PILR-beta is the activating counterpart to the inhibitory receptor immunoreceptor PILR-alpha which contains a tyrosine-based inhibitory motif (ITIM) (1). Mature human PILR-beta contains a V‑type Ig‑like extracellular domain (ECD) with a siglec-like fold, a single TM domain, and a truncated cytoplasmic tail (2, 3). The TM domain of PILR-beta contains a positively‑charged residue which interacts with immunoreceptor tyrosine-based activation (ITAM)-bearing adaptor molecules (2). The ECD of mature human PILR-beta shares 40% amino acid sequence identity with its mouse counterpart. PILR-beta is expressed on myeloid cells, such as natural killer, macrophage, and dendritic cells, as well as resident cells of the central nervous system, including microglial cells (2, 4). It is a binding partner for DAP12 and CD99 and has been shown to play an important role in innate immunity and inflammation (4-6). The PILR-alpha / beta pair have also been shown to regulate cell signaling via association with SHP-1 (7). Experiments studying the effects of S. aureus and T. gondii infections in mice have shown that up-regulation of PILR-beta led to significantly lower survival rates while knock-down of PILR-beta or activation of PILR-alpha resulted in significantly less inflammation and increased pathogen clearance (4, 5).
Wilson, M.D. et al. (2006) Physiol. Genomics 27:201.
Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
Lu, Q. et al. (2014) PNAS 111:8221.
Tato, C.M. et al. (2012) PLoS One 7:e31690.
Banerjee, A. et al. (2010) Infect. Immun. 78:1353.
Tabata, S. et al. (2008) J. Biol. Chem. 283:8893.
Mousseau, D.D. et al. (2000) J. Biol. Chem. 275:4467.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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