Product Specifications for Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF
Chinese Hamster Ovary cell line, CHO-derived human Olfactomedin-4/OLFM4 protein Asp21-Gln510, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
66-75 kDa, under reducting conditions
Measured by the ability of the immobilized protein to support the spreading of NIH3T3 mouse embryonic fibroblast cells Recombinant Human Olfactomedin-4/OLFM4 His-tag (Catalog # 10261-OL) immobilized at 0.5-1 μg/mL will significantly support cell spreading.
Scientific Data Examples for Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF
Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Human Olfactomedin-4/OLFM4 His-tag (Catalog # 10261-OL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 66-75 kDa on reducing gel.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Olfactomedin-4 (OLFM4), previously called GC-1 (G-CSF stimulated clone-1) is an approximately 64-72 kDa secreted glycoprotein that is a member of the Olfactomedin/Noelin family of protein (1-3). It is expressed in epithelial cells of the prostate, small intestine, colon, bone marrow, and in several cancers (4-7), and is up-regulated in epithelial cells during inflammation (8). Mature OLFM4 is a 490 amino acid (aa) protein that is secreted mainly in a polymeric form which is held together by disulfide bonds and carbohydrate interactions (2). Mature human OLFM4 shares 71% aa sequence identity with mature mouse and rat OLFM4. Olfactomedin-4 (OLFM4) interacts with cell surface lectins and cadherins, which helps cell adhesion and cell spreading of NIH3T3 and 293T/17 cells (9). OLFM4 plays a critical role in regulating progression of prostate cancer and is also novel biomarker for triple-negative breast cancer (10-11).
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Zhang, X. et al. (2004) Cancer Res. 64:2474.
Kobayashi, D. et al. (2007) Cancer Sci. 98:334.
Koshida, S. et al. (2007) Cancer Sci. 98:315.
Yu, L. et al. (2011) Int. J. Gynecol. Cancer 21:367.
Shinozaki, S. et al. (2001) Gut 48:623.
Liu, W. et al. (2006) Exp. Cell Res. 312:1785.
Xiong, B. et al. (2017) Biomed. Pharmacother. 86:67.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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