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Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF

Catalog # 10261-OL | R&D Systems, Inc. a Bio-Techne Brand
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10261-OL-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Olfactomedin-4/OLFM4 protein
Asp21-Gln510, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp21

Predicted Molecular Mass

56 kDa

SDS-PAGE

66-75 kDa, under reducting conditions

Activity

Measured by the ability of the immobilized protein to support the spreading of NIH3T3 mouse embryonic fibroblast cells
Recombinant Human Olfactomedin-4/OLFM4 His-tag (Catalog # 10261-OL) immobilized at 0.5-1 μg/mL will significantly support cell spreading.

Scientific Data Images for Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF

Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein SDS-PAGE

Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Olfactomedin-4/OLFM4 His-tag (Catalog # 10261-OL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 66-75 kDa on reducing gel.

Formulation, Preparation and Storage

10261-OL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Olfactomedin-4/OLFM4

Olfactomedin-4 (OLFM4), previously called GC-1 (G-CSF stimulated clone-1) is an approximately 64-72 kDa secreted glycoprotein that is a member of the Olfactomedin/Noelin family of protein (1-3). It is expressed in epithelial cells of the prostate, small intestine, colon, bone marrow, and in several cancers (4-7), and is up-regulated in epithelial cells during inflammation (8). Mature OLFM4 is a 490 amino acid (aa) protein that is secreted mainly in a polymeric form which is held together by disulfide bonds and carbohydrate interactions (2). Mature human OLFM4 shares 71% aa sequence identity with mature mouse and rat OLFM4. Olfactomedin-4 (OLFM4) interacts with cell surface lectins and cadherins, which helps cell adhesion and cell spreading of NIH3T3 and 293T/17 cells (9). OLFM4 plays a critical role in regulating progression of prostate cancer and is also novel biomarker for triple-negative breast cancer (10-11).

References

  1. Zhang, J. et al. (2002) Gene 283:83.
  2. Grover, P.K. et al. (2010) Cancer Metastasis Rev. 29:761.
  3. Guette, C. et al. (2015) Proteomics Clin. Appl. 9:58.
  4. Zhang, X. et al. (2004) Cancer Res. 64:2474.
  5. Kobayashi, D. et al. (2007) Cancer Sci. 98:334.
  6. Koshida, S. et al. (2007) Cancer Sci. 98:315.
  7. Yu, L. et al. (2011) Int. J. Gynecol. Cancer 21:367.
  8. Shinozaki, S. et al. (2001) Gut 48:623.
  9. Liu, W. et al. (2006) Exp. Cell Res. 312:1785.
  10. Xiong, B. et al. (2017) Biomed. Pharmacother. 86:67.
  11. Li, H. et al. (2015) Sci. Rep. 5:16974.

Alternate Names

GC1, GW112, OlfD, OLFM4, OLM4

Entrez Gene IDs

10562 (Human); 380924 (Mouse); 290409 (Rat)

Gene Symbol

OLFM4

UniProt

Product Documents for Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF

For research use only

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